Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate

doi:10.1016/j.bpc.2007.05.020

	Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate
	Li, Yiming ; Yin, Guowei ; Wei, Wei ; Wang, Hengbin ; Jiang, Shanhao ; Zhu, Dayuan ; Du, Weihong
刊名	BIOPHYSICAL CHEMISTRY
	2007-09
卷号	129 期号:2-3 页码:212-217
关键词	acetyleholinesterase Lycopodium alkaloids H-1 spin-lattice relaxation rate ligand-macromolecules interaction
ISSN号	0301-4622
DOI	10.1016/j.bpc.2007.05.020
文献子类	Article
英文摘要	In order to further understand the interaction processes between the Lycopodium alkaloids and acetylcholinesterase, the binding properties of N-acetyl Imperzine A (1), Imperzine B (2) and Imperzine F (3) with Torpediniforms Nacline acetylcholinesterase (TnAchE) were investigated by H-1 NMR methods. The nonselective, selective and double-selective spin-lattice relaxation rates were acquired in the absence and presence of TnAchE at a ratio of [ligand]/[protein] = 1:0.005. The selective relaxation rates show protons of 1-3 have dipole-dipole interaction with protons of TnAchE at the binding interface. The molecular rotational correlation time of bound ligands was calculated by double-selective relaxation rate at 298 K, which showed that 1-3 had high affinity with the protein. The results indicate that investigation of H-1 NMR relaxation data is a useful method to locate the new Lycopodium alkaloids as AchE inhibitors. C 2007 Elsevier B.V All rights reserved.
WOS关键词	TORPEDO-CALIFORNICA ACETYLCHOLINESTERASE ; SELECTIVE EXCITATION ; CROSS-RELAXATION ; HUPERZINE-A ; NMR ; INHIBITORS ; BINDING ; LIGANDS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000249151100012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273156]
专题	中国科学院上海药物研究所
通讯作者	Zhu, Dayuan
作者单位	1.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Li, Yiming,Yin, Guowei,Wei, Wei,et al. Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate[J]. BIOPHYSICAL CHEMISTRY,2007,129(2-3):212-217.
APA	Li, Yiming.,Yin, Guowei.,Wei, Wei.,Wang, Hengbin.,Jiang, Shanhao.,...&Du, Weihong.(2007).Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate.BIOPHYSICAL CHEMISTRY,129(2-3),212-217.
MLA	Li, Yiming,et al."Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate".BIOPHYSICAL CHEMISTRY 129.2-3(2007):212-217.