Protective effect of raloxifene on lipopolysaccharide and acid-induced acute lung injury in rats

doi:10.1111/j.1745-7254.2007.00637.x

	Protective effect of raloxifene on lipopolysaccharide and acid-induced acute lung injury in rats
	Guang-Ju Zhou ; Hong Zhang ; Sheng-De Zhi ; Guo-Ping Jiang ; Jing Wang ; Mao Zhang ; Jian-Xin Gan ; Shao-Wen Xu ; Guan-Yu Jiang
刊名	ACTA PHARMACOLOGICA SINICA
	2007-10
卷号	28 期号:10 页码:1585-1590
关键词	acute lung injury aspiration fluorodeoxy-glucose lipopolysaccharide microPET pulmonary edema raloxifene
ISSN号	1671-4083
DOI	10.1111/j.1745-7254.2007.00637.x
文献子类	Article
英文摘要	Aim: To evaluate the protective effect of oral raloxifene on acute lung injury. Methods: Thirty adult, male Sprague-Dawley rats each weighing 180-210 g were used and divided into 3 groups: the raloxifene-lipopolysaccharide (LPS)-HCl group (n=10), the LPS-raloxifene-HCl group (n=10), and the placebo group (n=10). All the rats were injected intraperitoneally (ip) with 5 mg/kg LPS, and raloxifene (30 mg/kg) was orally administered 1 h before and 14 h after LPS injection into the raloxifene-LPS-HCl and the LPS-raloxifene-HCl groups, respectively; the placebo group received nothing. Sixteen hours after LPS injection, all the animals were anesthetized and the femoral artery was cannulated. All the rats received a direct intratracheal (IT) injection of HCl (pH 1.2; 0.5 mL/kg). The mean arterial pressure (MAP) and blood gas concentrations were measured. Fifteen rats (5 in each group, respectively) underwent a micro positron emission tomography (microPET) scan of the thorax 4 h after HCl instillation. The wet/dry (W/D) weight ratio determination and histopathological examination were also performed. Results: The rats in the LPS-raloxifene-HCl group had a lower [F-18]fluorodeoxyglucose uptake compared with the rats in the placebo group (4.67 +/- 1.33 vs 9.01 +/- 1.58, respectively, P < 0.01). The rats in the LPS-raloxifene-HCl group also had a lower histological lung injury score (8.20 +/- 1.23 vs 12.6 +/- 0.97, respectively, P < 0.01) and W/D weight ratio (5.335 +/- 0.198 vs 5.886 +/- 0.257, respectively, P < 0. 01) compared to the placebo group. The rats in this group also showed better pulmonary gas exchange and more stable mean arterial pressure (MAP) compared to the placebo group. Conclusion: Raloxifene provides a significant protective effect on acute lung injury in rats induced first by LPS ip injection and then by HCl IT instillation.
WOS关键词	ACUTE RESPIRATORY-DISTRESS ; TOLL-LIKE RECEPTOR-4 ; HEMORRHAGIC-SHOCK ; FDG-PET ; INFLAMMATION ; ESTROGEN ; EXPRESSION ; INFECTION ; LEUKOCYTES ; PATTERN
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:3089044
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000249699600010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273133]
专题	中国科学院上海药物研究所
通讯作者	Guan-Yu Jiang
作者单位	1.Zhejiang Univ, Affiliated Hosp 2, Dept Emergency Med, Sch Med, Hangzhou 310009, Peoples R China 2.Zhejiang Univ, Res Inst Emergency Med, Hangzhou 310009, Peoples R China 3.Zhejiang Univ, Affiliated Hosp 2, Dept Nucl Med, Hangzhou 310009, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Drug Design & Discovery Ctr, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Guang-Ju Zhou,Hong Zhang,Sheng-De Zhi,et al. Protective effect of raloxifene on lipopolysaccharide and acid-induced acute lung injury in rats[J]. ACTA PHARMACOLOGICA SINICA,2007,28(10):1585-1590.
APA	Guang-Ju Zhou.,Hong Zhang.,Sheng-De Zhi.,Guo-Ping Jiang.,Jing Wang.,...&Guan-Yu Jiang.(2007).Protective effect of raloxifene on lipopolysaccharide and acid-induced acute lung injury in rats.ACTA PHARMACOLOGICA SINICA,28(10),1585-1590.
MLA	Guang-Ju Zhou,et al."Protective effect of raloxifene on lipopolysaccharide and acid-induced acute lung injury in rats".ACTA PHARMACOLOGICA SINICA 28.10(2007):1585-1590.