Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells

	Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells
	Wang Li; Jiang Wei; Zhu Xinzu
刊名	Chinese Journal of Anesthesiology
	2004
卷号	24 期号:6 页码:450-452
关键词	Ketamine PC12 cells Neurons Glutamic acid Apoptosis
ISSN号	0254-1416
其他题名	氯胺酮对谷氨酸引起神经元样嗜铬细胞瘤细胞株凋亡的影响
文献子类	Article
英文摘要	Objective To study the effects of ketamine on glutamate-induced apoptosis in neuronal cells using PC 12 pheochromocytoma cell line (provided by Chinese Academy of Phamacological Research) . Methods After being incubated in the culture medium containing 7S-NGF for 6 days. Over 95 % of the PC cells differentiated into neuron-type cells. The 7S-NGF induced dififerentiated neuronal PC 12 cells were seeded in 24-well plates pre-coated with poly-L-lysine(2 x 10s cells per well) .24 hours later the PC12 cells were exposed to glutamate 20 mmol/ L(group A); glutamate 20 mmol/L + ketamine 0.1 mmo/L (group B); glutamate 20 mmol/L + ketamine 1.0 mmol/L (group C) jglutamate 20 mmol/L + D-APS 100 funol/L(group D) and new culture medium containing no 7S-NGF(group E, control group) , and incubated for 18 hours .The viability of the cells was evaluated by the ability of the cells to reduce the tetragotium derivative MTT into a blue formagan salt. DNA fragmentation indicative of apoptosis was detected using TUNEL technique. Results in group A following incubation with glutamate 20 mmol/L for 18 h , at 37 V,, the viability was PC 12 cells was reduced to 37 % 6 % . However ketamine , when added to the culture medium to gather with glutamate , inhibited glutamate-induced cell death . The viability of PC12cell was 65 7% in group B an 99 10% in group C. Ketamine appeared to attenuate the apoptotic process, because the number of the apoptotic cell bodies, determinated by YUNEL was also reduced by ketamine, with only 15 ~ 20% of neuronal cells staining positive after exposure to 20 mmol/L glutamate .The difference between group A and C was very significant ( P < 0.01) .In addition D-APS 100 fimol /L ( a competitive antagonist) also exerted significant protective effects on neuronal PC 12 cells. Conclusion Ketamine protects neuronal PC 12 cells from glutamate neurotoxicity induced apoptosis.
WOS研究方向	Surgery (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:1661276
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268365]
专题	中国科学院上海药物研究所
作者单位	Department of Anesthesiolagy, Sixth People's Hospital, Shanghai Jiaotong University, 200233.
推荐引用方式 GB/T 7714	Wang Li,Jiang Wei,Zhu Xinzu. Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells[J]. Chinese Journal of Anesthesiology,2004,24(6):450-452.
APA	Wang Li,Jiang Wei,&Zhu Xinzu.(2004).Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells.Chinese Journal of Anesthesiology,24(6),450-452.
MLA	Wang Li,et al."Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells".Chinese Journal of Anesthesiology 24.6(2004):450-452.