Characterization of a fluorouracil-resistant human gastric carcinoma cell line and its morphological behavior

	Characterization of a fluorouracil-resistant human gastric carcinoma cell line and its morphological behavior
	Yue, X F; Han, J X; Dai, Z Q; Liu, M Z; Yang, W Y; Xu, J Y; Zhou, J J; Chen, L J; Zhang, S Y
	1993-11
卷号	14 Suppl
页码	S1-4
英文摘要	A fluorouracil (FU)-resistant human carcinoma cell line (SGC-7901/R) was established in culture by progressively increasing the concentration of FU from 50 ng.ml-1 to 2.5 mg.ml-1. The cell line has been successfully subcultured for more than 150 passages during more than 2 years. Its degree of FU resistance was 139-fold vs that of FU sensitive cell line (SGC-7901/S), and the resistant phenotype was stable when cells were cultured for 23 passages in FU-free medium. The doubling time was 17.3 and 25.6 h for resistant cells and parental cells, respectively. Swiss (nu/nu) nude mice were used for the in vivo experiment, the FU-resistant cell line also exhibited resistance to FU and cross-resistance to mitomycin C. FU inhibited markedly the incorporation of [3H]UR into sensitive cells and only showed a 31.6% inhibition with FU 100 micrograms.ml-1 in resistant cells. For the incorporation of [3H]TdR into DNA, inhibitory rates were seen with different concentrations of FU in resistant cells. By morphologic observation, SGC-7901/R cells showed little secretion but without any tendency to glandular pattern. Their nuclei were allotype with enlarged perinuclear space and a few intranuclear pseudoinclusions. The mitotic phase of cells was found frequently. The phenotype of resistant cell line can be deduced more malignant than that of parental cell line.
会议录	Zhongguo yao li xue bao = Acta pharmacologica Sinica
文献子类	Meeting Abstract;Article
语种	中文
内容类型	会议论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266925]
专题	中国科学院上海药物研究所
作者单位	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Yue, X F,Han, J X,Dai, Z Q,et al. Characterization of a fluorouracil-resistant human gastric carcinoma cell line and its morphological behavior[C]. 见:.