Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines

doi:10.1016/j.ijbiomac.2016.05.046

	Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines
	Wu, Yajun 1,3; Li, Shuai 1; Li, Haixia 1; Zhao, Chunzhi 2; Ma, Hao 1; Zhao, Xiunan 1; Wu, Junhua 1; Liu, Kunlu 1; Shan, Junjie 1,3; Wang, Yuxia 1
刊名	International journal of biological macromolecules
	2016-10
卷号	91 页码:248-57
ISSN号	1879-0003
DOI	10.1016/j.ijbiomac.2016.05.046
文献子类	Article
英文摘要	Poria cocos has a long history of medicinal use in China. Polysaccharides and their derivatives in the medicine exhibit many beneficial biological activities including anticancer, anti-inflammatory, antioxidant and antiviral activities. In this study, a new polysaccharide (PCP-II) was isolated from sclerotium of Poria cocos. Its physico-chemical characters were identified and its adjuvant activity was investigated in mice co-immunized with H1N1 influenza vaccine and hepatitis B surface antigen (HBsAg). The results revealed that PCP-II has a molecular weight of 29.0kDa. It was composed of fucose, mannose, glucose and galactose in molar ration of 1.00:1.63:0.16:6.29 respectively. Pharmacological data demonstrated that PCP-II increased antigen-specific antibody levels in mice immunized with influenza vaccine. PCP-II also elicited anti-HBsAg antibodies at significantly higher titers and generated robust and durable immunity compared to mice immunized with HBsAg-alum following two administrations. PCP-II improved proliferation of splenocytes, stimulated IL-12p70 and TNF-α productions in dendritic cells and macrophages respectively. These results suggested that PCP-II-adjuvanted vaccines enhanced humoral and cellular immunity. PCP-II could be developed as an efficacious adjuvant in human and animal vaccines.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266625]
专题	中国科学院上海药物研究所
通讯作者	Wu, Yajun; Li, Shuai; Li, Haixia; Zhao, Chunzhi; Ma, Hao; Zhao, Xiunan; Wu, Junhua; Liu, Kunlu; Shan, Junjie; Wang, Yuxia
作者单位	1.Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China; 2.Department of Pharmacy, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China 3.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China;
推荐引用方式 GB/T 7714	Wu, Yajun,Li, Shuai,Li, Haixia,et al. Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines[J]. International journal of biological macromolecules,2016,91:248-57.
APA	Wu, Yajun.,Li, Shuai.,Li, Haixia.,Zhao, Chunzhi.,Ma, Hao.,...&Wang, Yuxia.(2016).Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines.International journal of biological macromolecules,91,248-57.
MLA	Wu, Yajun,et al."Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines".International journal of biological macromolecules 91(2016):248-57.