Regulating the Behavior of Human Gingival Fibroblasts by sp(2) Domains in Reduced Graphene Oxide

doi:10.1021/acsbiomaterials.9b00497

	Regulating the Behavior of Human Gingival Fibroblasts by sp(2) Domains in Reduced Graphene Oxide
	Wang, Lanyu; Qiu, Jiajun; Guo, Jingshu; Wang, Donghui; Qian, Shi; Cao, Huiliang; Liu, Xuanyong
刊名	ACS BIOMATERIALS SCIENCE & ENGINEERING
	2019-12-30
卷号	5 期号:12 页码:6414
关键词	human gingival fibroblasts soft tissue sealing reduced graphene oxide sp(2) domains
ISSN号	2373-9878
DOI	10.1021/acsbiomaterials.9b00497
文献子类	Article
英文摘要	Long-term function of dental implants relies on not only stable osseointegration but also strong soft tissue-sealing ability. Ideal soft tissue sealing around implants is an effective protective barrier between the external environment and alveolar bone, preventing the invasion of bacteria that is considered as a vital trigger of irreversible marginal bone loss. Carbon-based materials have been reported to be beneficial to soft tissue sealing, which can be regulated through the hybridization type of carbon atoms (sp(2) or sp(3)), but its internal mechanism is still not clear. In this work, graphene oxide with both sp(2) - and sp(3)-hybridized carbons was electrophoretic deposited on titanium and reduced to regulate the hybridization type of carbon atoms to investigate its effect and possible mechanism on human gingival fibroblasts (HGFs). X- ray photoelectron spectroscopy and Raman mapping test show the increase of sp(2) domain content and the decrease of their size after reduction. Through computer simulation, the possible mechanism of the decrease of sp(2) domain size was proposed. In vitro studies disclose that the HGFs exhibit higher proliferation rate, better adhesion, and migration ability with the increase of sp(2) domains and the decrease of their sizes. It may be due to the amount and size of sp(2) domains that synergistically regulate the amount and properties of adsorbed proteins, thereby influencing the cellular behaviors of HGFs. Our results may offer a different perspective on material designing and academic research to enhance the soft tissue integration of implants.
WOS关键词	CARBON NANOTUBES ; IN-VITRO ; AQUEOUS DISPERSIONS ; GRAPHITE OXIDE ; TITANIUM ; REDUCTION ; NANOSHEETS ; MECHANISM ; SURFACE ; FILMS
WOS研究方向	Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/27458]
专题	中国科学院上海硅酸盐研究所
推荐引用方式 GB/T 7714	Wang, Lanyu,Qiu, Jiajun,Guo, Jingshu,et al. Regulating the Behavior of Human Gingival Fibroblasts by sp(2) Domains in Reduced Graphene Oxide[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2019,5(12):6414.
APA	Wang, Lanyu.,Qiu, Jiajun.,Guo, Jingshu.,Wang, Donghui.,Qian, Shi.,...&Liu, Xuanyong.(2019).Regulating the Behavior of Human Gingival Fibroblasts by sp(2) Domains in Reduced Graphene Oxide.ACS BIOMATERIALS SCIENCE & ENGINEERING,5(12),6414.
MLA	Wang, Lanyu,et al."Regulating the Behavior of Human Gingival Fibroblasts by sp(2) Domains in Reduced Graphene Oxide".ACS BIOMATERIALS SCIENCE & ENGINEERING 5.12(2019):6414.