Lithium-containing biomaterials stimulate bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis

doi:10.1016/j.biomaterials.2018.11.007

	Lithium-containing biomaterials stimulate bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis
	Liu, Lu; Liu, Yaqin; Feng, Chun; Chang, Jiang; Fu, Runqing; Wu, Tingting; Yu, Fei; Wang, Xiaoting; Xia, Lunguo; Wu, Chengtie
刊名	BIOMATERIALS
	2019-02-01
卷号	192 页码:523
关键词	Exosome MiR-130a/PTEN/AKT pathway Lithium Biomaterial Angiogenesis
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2018.11.007
文献子类	Article
英文摘要	The chemical signals of biomaterials could influence bone marrow stromal cells (BMSCs)-endothelial cells (ECs) communication during vascularized bone regeneration. However, the underlying mechanisms still remain unknown. Exosomes, a series of extracellular vesicles, have recently emerged as potential paracrine mediators in cell-cell communication. However, whether exosomes and exosomal microRNAs (miRNAs) are involved in the chemical signals of biomaterials-modulated BMSCs-ECs communication are unknown. Hence, in the present study, a model Li-incorporated bioactive glass ceramic (Li-BGC) was applied to explore the chemical signals of biomaterials mediated cell-cell communication between BMSCs and ECs. Our results showed that Li-BGC directly promoted the pro-angiogenic capability of HUVECs in vitro and new blood vessel ingrowth in vivo. Moreover, Li-BGC activated Wnt/beta-catenin, AKT and NF-kappa B signaling pathways, while AKT signaling pathway might function as the upstream of Wnt/beta-catenin and NF-kappa B signaling pathways. More importantly, Li-BGC further facilitated the pro-angiogenic capacity of HUVECs by eliciting the expression of exosomal pro-angiogenic miR-130a in BMSCs-derived exosomes, which subsequently leading to the downregulation of PTEN protein and activation of AKT pathway, ultimately resulting in the elevated proliferation, migration and tube formation of endothelial cells, as well as the upregulated expression of pro-angiogenic genes. Our findings may provide new insights into the regulatory roles of the chemical signals of biomaterials in BMSCs-ECs communication via stimulating exosomal miR-130a secretion and PTEN/AKT signaling pathway in the angiogenic process of bone remodelling.
WOS研究方向	Engineering ; Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/27369]
专题	中国科学院上海硅酸盐研究所
推荐引用方式 GB/T 7714	Liu, Lu,Liu, Yaqin,Feng, Chun,et al. Lithium-containing biomaterials stimulate bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis[J]. BIOMATERIALS,2019,192:523.
APA	Liu, Lu.,Liu, Yaqin.,Feng, Chun.,Chang, Jiang.,Fu, Runqing.,...&Fang, Bing.(2019).Lithium-containing biomaterials stimulate bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis.BIOMATERIALS,192,523.
MLA	Liu, Lu,et al."Lithium-containing biomaterials stimulate bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis".BIOMATERIALS 192(2019):523.