The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge

doi:10.1016/j.fsi.2019.08.004

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge
	Wang, Yilong 4,5,6; Wang, Baojie 5,6; Shao, Xuqing 1; Shao, Jianchun 4,5,6; Liu, Mei 5,6; Wang, Mengqiang3,5,6 ; Wang, Lei 2,5,6
刊名	FISH & SHELLFISH IMMUNOLOGY
	2019-10-01
卷号	93 页码:517-530
关键词	Rearing density Vibrio parahaemolyticus E1 Transcriptome Histopathology Antioxidant status
ISSN号	1050-4648
DOI	10.1016/j.fsi.2019.08.004
通讯作者	Wang, Mengqiang(wangmengqiang@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
英文摘要	Rearing density and disease management are considered as pivotal factors determining shrimp farm productivity and profitability. To systematically investigate the potential mechanisms for density-related differences between disease susceptibility and rearing densities, we conducted comparative transcriptome analysis of the molecular differences between hepatopancreas and intestine of Litopenaeus vannamei under two different rearing densities (800- and 400- shrimp/m(3)) for 15 d and further analyzed the differences in immune response to Vibrio parahaemolyticus E1 (VPE1) raised under two density conditions. Totally 45 different expression genes (DEGs) were identified in the hepatopancreas under two different rearing densities, the DEGs were grouped into four processes or pathways related to animal immune system. Then, exposure to the VPE1 resulted in 639 DEGs, involved into fourteen immune related processes or pathways. In the intestine, seventeen processes or pathways related to the immune system were identified among the 5470 DEGs under two different rearing densities. 279 DEGs were identified post VPE1 challenge, classified into five processes or pathways associated with the immune system. Meanwhile, the results of growth performance, histopathology and the activities of antioxidant enzymes in the hepatopancreas and intestines of shrimp showed that high density decreased weight gain rate (63.20 +/- 1.67% and 18.73 +/- 3.35% in the high and low rearing density groups, respectively), severely destroyed the histopathology and inhibited the antioxidant enzymes activities. This study demonstrated that rearing density in L. vannamei significantly impacts susceptibility to the VPE1, via altered transcriptional challenge responses, and thus higher mortality due to disease.
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-2] ; Science and Technology Development Fund Project of Shinan district, Qingdao city, China[2018-4-001-ZH]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000488998300058
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/163235]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Mengqiang; Wang, Lei
作者单位	1.Shandong Cigna Detect Technol Co Ltd, Qingdao 266237, Shandong, Peoples R China 2.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China 3.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yilong,Wang, Baojie,Shao, Xuqing,et al. The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:517-530.
APA	Wang, Yilong.,Wang, Baojie.,Shao, Xuqing.,Shao, Jianchun.,Liu, Mei.,...&Wang, Lei.(2019).The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge.FISH & SHELLFISH IMMUNOLOGY,93,517-530.
MLA	Wang, Yilong,et al."The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge".FISH & SHELLFISH IMMUNOLOGY 93(2019):517-530.