Poly-adenine-mediated spherical nucleic acids for strand displacement based DNA/RNA detection

doi:10.1016/j.bios.2018.12.003

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Poly-adenine-mediated spherical nucleic acids for strand displacement based DNA/RNA detection
	Wang, L ; Zhang, H ; Wang, CG ; Xu, Y ; Su, J ; Wang, X ; Liu, XX ; Feng, DZ ; Wang, LH ; Zuo, XL
刊名	BIOSENSORS & BIOELECTRONICS
	2019
卷号	127 期号:-页码:85—91
关键词	GOLD NANOPARTICLES COLORIMETRIC DETECTION MOLECULAR BEACONS NONTHIOLATED DNA CHAIN-REACTION MESSENGER-RNA PROBES MICRORNA SEQUENCE KINETICS
ISSN号	0956-5663
DOI	10.1016/j.bios.2018.12.003
文献子类	期刊论文
英文摘要	DNA-gold nanoparticles (AuNPs) conjugate is one of the most versatile bionanomaterials for biomedical and clinical diagnosis. However, to finely tune the hybridization ability and precisely control the orientation and conformation of surface-tethered oligonucleotides on AuNPs remains a hurdle. In this work, we developed a poly adenine-mediated spherical nucleic acid (polyA-mediated SNA) strategy by assembling di-block DNA probes on gold nanoparticles (AuNPs) to spatially control interdistance and hybridization ability of oligonucleotides on AuNPs. By modulating length of poly A bound on the SNA with different degrees of constructing, we presented significant improved biosensing performance including high hybridization efficiency, and expanded dynamic range of analytes with more sensitive detection limit. Furthermore, this polyA design could facilitate the programmable detection for DNA in serum environment and simultaneous multicolor detection of three different microRNAs associated with pancreatic carcinoma. The demonstration of the link between modulation of SNA assembly strategy and biodetection capability will increase the development of high performance diagnostic tools for translational biomedicine.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31760]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, Shanghai 201800, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Med, Sch Chem & Chem Engn, Inst Mol Med,Renji Hosp, Shanghai 200240, Peoples R China 5.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201220, Peoples R China;
推荐引用方式 GB/T 7714	Wang, L,Zhang, H,Wang, CG,et al. Poly-adenine-mediated spherical nucleic acids for strand displacement based DNA/RNA detection[J]. BIOSENSORS & BIOELECTRONICS,2019,127(-):85—91.
APA	Wang, L.,Zhang, H.,Wang, CG.,Xu, Y.,Su, J.,...&Mi, XQ.(2019).Poly-adenine-mediated spherical nucleic acids for strand displacement based DNA/RNA detection.BIOSENSORS & BIOELECTRONICS,127(-),85—91.
MLA	Wang, L,et al."Poly-adenine-mediated spherical nucleic acids for strand displacement based DNA/RNA detection".BIOSENSORS & BIOELECTRONICS 127.-(2019):85—91.