Poly-adenine-mediated fluorescent spherical nucleic acid probes for live-cell imaging of endogenous tumor-related mRNA

doi:10.1016/j.nano.2018.05.006

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	Poly-adenine-mediated fluorescent spherical nucleic acid probes for live-cell imaging of endogenous tumor-related mRNA
	Zhu, D ; Zhao, DX ; Huang, JX ; Zhu, Y ; Chao, J ; Su, S ; Li, J ; Wang, LH ; Shi, JY ; Zuo, XL
刊名	NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
	2018
卷号	14 期号:6 页码:1797-1807
关键词	LIVING CELLS GOLD NANOPARTICLES MOLECULAR-DETECTION DNA NANOSTRUCTURE NANO-FLARES C-MYC CANCER OLIGONUCLEOTIDE FUNCTIONALIZATION QUANTIFICATION
ISSN号	1549-9634
DOI	10.1016/j.nano.2018.05.006
文献子类	期刊论文
英文摘要	Identification of tumor-related mRNA in living cells hold great promise for early cancer diagnosis and pathological research. Herein, we present poly-adenine (polyA)-mediated fluorescent spherical nucleic acid (FSNA) probes for intracellular mRNA detection with regulable sensitivities by programmably adjusting the loading density of DNA on gold nano-interface. Gold nanoparticles (AuNPs) functionalized with polyA-tailed recognition sequences were hybridized to fluorescent "reporter" strands to fabricate fluorescence-quenched FSNA probes. While exposed to target gene, the "reporter" strands were released from FSNA through strand displacement and fluorescence was recovered. With polyA20 tail as the attaching block, the detection limit of FSNA probes was calculated to be 0.31 nM, which is -55 fold lower than that of thiolated probes without surface density regulation. Quantitative intracellular mRNA detection and imaging could be achieved with polyA-mediated FSNA probes within 2 hours, indicating their application potential in rapid and sensitive intracellular target imaging. (C) 2018 Elsevier Inc. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31060]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai, Peoples R China 2.Nanjing Univ Posts & Telecommun, Key Lab Organ Elect & Informat Displays, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, IAM, 9 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China 3.Nanjing Univ Posts & Telecommun, Jiangsu Key Lab Biosensors, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, IAM, 9 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China 4.Nanjing Univ Posts & Telecommun, Coll Geog & Biol Informat, 9 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China 5.Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 6.Chinese Acad Sci, Bioimaging Ctr, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 7.UCB Pharma, Slough, Berks, England 8.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhu, D,Zhao, DX,Huang, JX,et al. Poly-adenine-mediated fluorescent spherical nucleic acid probes for live-cell imaging of endogenous tumor-related mRNA[J]. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,2018,14(6):1797-1807.
APA	Zhu, D.,Zhao, DX.,Huang, JX.,Zhu, Y.,Chao, J.,...&Wang, LH.(2018).Poly-adenine-mediated fluorescent spherical nucleic acid probes for live-cell imaging of endogenous tumor-related mRNA.NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,14(6),1797-1807.
MLA	Zhu, D,et al."Poly-adenine-mediated fluorescent spherical nucleic acid probes for live-cell imaging of endogenous tumor-related mRNA".NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 14.6(2018):1797-1807.