In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25

doi:10.1016/j.resmic.2018.08.003

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25
	Liu, LZ ; Nie, ZY ; Yang, Y ; Pan, X ; Xia, X ; Zhou, YH ; Xia, JL ; Zhang, LJ ; Zhen, XJ ; Yang, HY
刊名	RESEARCH IN MICROBIOLOGY
	2018
卷号	169 期号:10 页码:590-597
关键词	EXTRACELLULAR THIOL-GROUPS SULFOBACILLUS-THERMOSULFIDOOXIDANS DIFFERENTIAL EXPRESSION SURFACE-PROPERTIES ELEMENTAL SULFUR FERROUS-IONS SUBSTANCES ADHESION ACID EPS
ISSN号	0923-2508
DOI	10.1016/j.resmic.2018.08.003
文献子类	期刊论文
英文摘要	For the first time, synchrotron radiation (SR) -based carbon Kedge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S-0, FeSO4). The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount. XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (-C- O, -C-0 and -C-N). The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S-0-acclimated cells. The FeSO4-acclimated cells contained the highest proportion of proteins, while the S-0-acclimated cells contained more lipids and polysaccharides. The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values. These differences are caused by the inconsistencies of measurement depth between XANES and the latter two characterization methods. (C) 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/30985]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.CSIRO Mineral Resources, Clayton, Vic 3169, Australia 2.Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China 3.Cent S Univ, Minist Educ China, Key Lab Biomet, Changsha 410083, Hunan, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China 5.Northeastern Univ, Sch Met, Shenyang 110819, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Liu, LZ,Nie, ZY,Yang, Y,et al. In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25[J]. RESEARCH IN MICROBIOLOGY,2018,169(10):590-597.
APA	Liu, LZ.,Nie, ZY.,Yang, Y.,Pan, X.,Xia, X.,...&Yang, HY.(2018).In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.RESEARCH IN MICROBIOLOGY,169(10),590-597.
MLA	Liu, LZ,et al."In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25".RESEARCH IN MICROBIOLOGY 169.10(2018):590-597.