experimentalstudyofcellmigrationandfunctionaldifferentiationoftransplantedneuralstemcellscolabeledwithsuperparamagneticironoxideandbrduinanischemicratmodel

	experimentalstudyofcellmigrationandfunctionaldifferentiationoftransplantedneuralstemcellscolabeledwithsuperparamagneticironoxideandbrduinanischemicratmodel
	WENZHEN ZHU 1; XIANG LI 1; JIANPIN QI 1; ZHOUPING TANG 2; WEI WANG 2; LI WEI 3; HAO LEI 3
刊名	biomedicalandenvironmentalsciences
	2008
卷号	21 期号:5 页码:420
ISSN号	0895-3988
英文摘要	Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide (SPIO) and bromodeoxyuridine (Brdu) using the 4.7T MR system and to study the cell differentiation with immuno-histochemical method in ischemic rats. Methods Rat neural stem cells (NSCs)co-labelled with SPIO mediated by poly-L-lysine and bromodeoxyuridine (BrdU) were transplanted into the unaffected side of rat brain with middle cerebral artery occlusion (MCAO). At weeks 1, 2, 3, 4, 5, and 6 after MCAO, migration of the labelled cells was monitored by MRI. At week 6, the rats were killed and their brain tissue was cut according to the migration site of transplanted cells indicated by MRI and subjected to Prussian blue staining and immunohistochemical staining to observe the migration and differentiation of the transplanted NSCs. Results Three weeks after transplantation, the linear hypointensity area derived from the migration of labelled NSCs was observed by MRI in the corpus callosum adjacent to the injection site. Six weeks after the transplantation, the linear hypointensity area was moved toward the midline along the corpus callosum. MRI findings were confirmed by Prussian blue staining and immunohistochemical staining of the specimen at week 6 after the transplantation. Flourescence co-labelled immunohistochemical methods demonstrated that the transplanted NSCs could differentiate into astrocytes and neurons. Conclusions MRI can monitor the migration of SPIO-labelled NSCs after transplantation in a dynamical and non-invasive manner. NSCs transplanted into ischemic rats can differentiate into astrocytes and neurons during the process of migration.
资助项目	[National Natural Science Foundation of China]
语种	英语
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/16380]
专题	中国科学院武汉物理与数学研究所
作者单位	1.华中科技大学 2.华中科技大学同济医学院 3.中国科学院武汉物理与数学研究所
推荐引用方式 GB/T 7714	WENZHEN ZHU,XIANG LI,JIANPIN QI,et al. experimentalstudyofcellmigrationandfunctionaldifferentiationoftransplantedneuralstemcellscolabeledwithsuperparamagneticironoxideandbrduinanischemicratmodel[J]. biomedicalandenvironmentalsciences,2008,21(5):420.
APA	WENZHEN ZHU.,XIANG LI.,JIANPIN QI.,ZHOUPING TANG.,WEI WANG.,...&HAO LEI.(2008).experimentalstudyofcellmigrationandfunctionaldifferentiationoftransplantedneuralstemcellscolabeledwithsuperparamagneticironoxideandbrduinanischemicratmodel.biomedicalandenvironmentalsciences,21(5),420.
MLA	WENZHEN ZHU,et al."experimentalstudyofcellmigrationandfunctionaldifferentiationoftransplantedneuralstemcellscolabeledwithsuperparamagneticironoxideandbrduinanischemicratmodel".biomedicalandenvironmentalsciences 21.5(2008):420.