Identification of the triacylglycerol lipase in the chloroplast envelope of the diatom Phaeodactylum tricornutum | |
Li, Xiaolong1,2; Pan, Yufang2; Hu, Hanhua2 | |
刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS |
2018-07-01 | |
卷号 | 33期号:1页码:440-447 |
关键词 | Phaeodactylum tricornutum Triacylglycerol Lipase Alga biofuel Polyunsaturated fatty acids |
ISSN号 | 2211-9264 |
DOI | 10.1016/j.algal.2018.06.023 |
英文摘要 | Triacylglycerol (TAG) accumulation mechanisms under stress have been intensively investigated in recent years in microalgae and efforts aiming to increase TAG synthesis have been made through gene engineering. However, only a few studies report the contribution of lipid breakdown to TAG accumulation in microalgae. In this study, a putative TAG lipase OmTGL in P. tricornutum was identified based on conserved domain and phylogenetic analysis. OmTGL is different from those known lipases and localized in the third outermost plastid membrane. OmTGL mRNA levels exhibited the consistent decrease during TAG accumulation and knockdown of OmTGL considerably enhanced neutral lipid content detected by Nile Red assay at the stationary growth phase, and TAGs detected by thin layer chromatography (TLC) increased by 1.2-1.4 folds in the OmTGL RNAi lines at day 6. Although C20:5 omega 3 fatty acid in the total lipid did not change in OmTGL mutants compared with the wild-type, its content in TAGs increased by 68-70%, indicating that TAG enriched with C20:5 was degraded under nitrogen stress and the degraded fatty acids and diacylglycerol (DAG) might feed into the biosynthesis of plastid membrane lipids. Therefore, the lipase is important for maintaining lipid homeostasis in plastid, and a slight slower growth and decreased glycolipids and phospholipids have also been found in the mutants. This study shows that this lipase is a good target for regulating not only TAG accumulation but also long-chain polyunsaturated fatty acids (LC-PUFA) flux to TAGs. |
资助项目 | National Natural Science Foundation of China[41576144] ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China[OF2017N003] |
WOS关键词 | SACCHAROMYCES-CEREVISIAE ; NUCLEAR TRANSFORMATION ; LIPID PARTICLES ; TRIAD FORMS ; CARBON FLUX ; ACCUMULATION ; MICROALGAE ; REDUCTASE ; PROTEINS ; ENCODES |
WOS研究方向 | Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000438910600050 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; 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National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; 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National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; Open Fund of Laboratory for Marine Biology and Biotechnology of the Qingdao National Laboratory for Marine Science and Technology, Qingdao, China ; 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内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/34537] |
专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
通讯作者 | Hu, Hanhua |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Xiaolong,Pan, Yufang,Hu, Hanhua. Identification of the triacylglycerol lipase in the chloroplast envelope of the diatom Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2018,33(1):440-447. |
APA | Li, Xiaolong,Pan, Yufang,&Hu, Hanhua.(2018).Identification of the triacylglycerol lipase in the chloroplast envelope of the diatom Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,33(1),440-447. |
MLA | Li, Xiaolong,et al."Identification of the triacylglycerol lipase in the chloroplast envelope of the diatom Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 33.1(2018):440-447. |
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