Effects of a bone graft substitute consisting of porous gradient HA/ZrO2 and gelatin/chitosan slow-release hydrogel containing BMP-2 and BMSCs on lumbar vertebral defect repair in rhesus monkey

CORC > 昆明动物研究所 > 昆明动物研究所 > 中国科学院昆明灵长类研究中心

	Effects of a bone graft substitute consisting of porous gradient HA/ZrO2 and gelatin/chitosan slow-release hydrogel containing BMP-2 and BMSCs on lumbar vertebral defect repair in rhesus monkey
	Shao RX 1,2; Quan RF*1; Wang D 2; Lv LB 3; Xu CY 3; Wei XC 4; Wan JF 5; Yang DS 6; Wang T 1; Du WB 1
刊名	Journal of Tissue Engineering and Regenerative Medicine
	2017
卷号	*期号:页码:Accepted
关键词	Bmp-2 Bmscs Bone-graft Substitute Gelatin/chitosan Hydrogel Ha/zro2 Composite Tissue Engineering
英文摘要	Dense biomaterial plays an important role in bone replacement. However, it fails to induce bone-cell migration into graft material. In the present study, a novel bone-graft substitute (BGS) consisting of porous gradient HA/ZrO₂ composite (PGHC) and gelatin/chitosan slow-release hydrogel containing BMP-2 and BMSCs was designed and prepared to repair lumbar vertebral defects. The morphological characteristics of the BGS evaluated by scanning electron microscope showed that it had a three-dimensional network structure with uniformly distributed chitosan microspheres on the surfaces of the graft material and the interior of the pores. Then, BGS (group A), PGHC (group B), or autologous bone (group C) was implanted into lumbar vertebral body defects in a total of 24 healthy rhesus monkeys. After 8 and 16 weeks, anteroposterior and lateral radiographs of the lumbar spine, micro-computed tomography (micro-CT), histomorphometry, biomechanical testing, and biochemical testing for bone-matrix markers, including type I collagen, osteocalcin, osteopontin, basic fibroblast growth factor, alkaline phosphatase, and vascular endothelial growth factor, were performed to examine the reparative efficacy of the BGS and PGHC. The BGS displayed excellent ability to repair the lumbar vertebral defect in the rhesus monkey. Radiography, micro-CT scanning, and histomorphological characterization showed that the newly formed bone volume in the interior of the pores in the BGS was significantly higher than in the PGHC. The results of biomechanical testing indicated that the vertebral body compression strength of the PGHC implant was lower than the other implants. RT-PCR and western-blot analyses showed that the expression of bone-related proteins in the BGS implant was significantly higher than in the PGHC implant. The BGS displayed reparative effects similar to autologous bone. Therefore, BGS use in vertebral bone defect repair appears promising.
语种	英语
资助机构	This work was supported by the Major Science and Technology Projects in Zhejiang Province [2014C13SA190012]. We also thank the School of Materials Science and Engineering (Shanghai University) for preparing the novel porous gradient HA/ZrO2 engineered BGS and providing information on its structure. ; This work was supported by the Major Science and Technology Projects in Zhejiang Province [2014C13SA190012]. We also thank the School of Materials Science and Engineering (Shanghai University) for preparing the novel porous gradient HA/ZrO2 engineered BGS and providing information on its structure. ; This work was supported by the Major Science and Technology Projects in Zhejiang Province [2014C13SA190012]. We also thank the School of Materials Science and Engineering (Shanghai University) for preparing the novel porous gradient HA/ZrO2 engineered BGS and providing information on its structure. ; This work was supported by the Major Science and Technology Projects in Zhejiang Province [2014C13SA190012]. We also thank the School of Materials Science and Engineering (Shanghai University) for preparing the novel porous gradient HA/ZrO2 engineered BGS and providing information on its structure.
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/12086]
专题	昆明动物研究所_中国科学院昆明灵长类研究中心
通讯作者	spinequan@yahoo.com
作者单位	1.Department of Orthopedics, Affiliated Jiangnan Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, 311201, China 2.Department of Orthopedics, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang Province, 310007, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan Province, 650223, China 4.School of Materials Science and Engineering, Shanghai University, Shanghai, 200072, China 5.Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058, China 6.Department of Orthopedics, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, 310009, China
推荐引用方式 GB/T 7714	Shao RX,Quan RF,Wang D,et al. Effects of a bone graft substitute consisting of porous gradient HA/ZrO2 and gelatin/chitosan slow-release hydrogel containing BMP-2 and BMSCs on lumbar vertebral defect repair in rhesus monkey[J]. Journal of Tissue Engineering and Regenerative Medicine,2017,():Accepted.
APA	Shao RX.,Quan RF.,Wang D.,Lv LB.,Xu CY.,...&spinequan@yahoo.com.(2017).Effects of a bone graft substitute consisting of porous gradient HA/ZrO2 and gelatin/chitosan slow-release hydrogel containing BMP-2 and BMSCs on lumbar vertebral defect repair in rhesus monkey.Journal of Tissue Engineering and Regenerative Medicine,(),Accepted.
MLA	Shao RX,et al."Effects of a bone graft substitute consisting of porous gradient HA/ZrO2 and gelatin/chitosan slow-release hydrogel containing BMP-2 and BMSCs on lumbar vertebral defect repair in rhesus monkey".Journal of Tissue Engineering and Regenerative Medicine *.(2017):Accepted.