Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing

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	Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing
	Yang, LX; Yang, YD; Yuan, JM; Sun, Y; Dai, JP; Su, B
刊名	CEREBRAL CORTEX
	2019
卷号	29 期号:2 页码:838-851
ISSN号	1047-3211
英文摘要	The von Economo neurons (VENs) are specialized large bipolar projection neurons with restricted distribution in the human brain, and they are far more abundant in humans than in non-human primates. However, VEN functions remain elusive due to the difficulty of isolating VENs and dissecting their connections in the brain. Here, we combined laser-capture-microdissection with RNA sequencing to describe the transcriptomic profile of VENs from human anterior cingulate cortex (ACC). Using pyramidal neurons as reference cells, we identified 344 genes with VEN-associated expression differences, including 215 higher and 129 lower expression genes. Functional enrichment and protein-protein interaction network analyses showed that these genes with VEN-associated expression differences are involved in VEN morphogenesis and functions, such as dendrite branching and axon myelination, and many of them are associated with human social-emotional disorders. With the use of in situ hybridization and immunohistochemistry assays, we validated four novel VEN markers (VAT1L, CHST8, LYPD1, and SULF2). Collectively, we generated a full-spectrum expression profile of VENs from human ACC, greatly enlarging the pool of genes with VEN-associated expression differences that can help researchers to understand the role of VENs in normal and disordered human brains.
语种	英语
内容类型	期刊论文
源URL	[http://ir.kiz.ac.cn:8080/handle/152453/12548]
专题	昆明动物研究所_比较基因组学
推荐引用方式 GB/T 7714	Yang, LX,Yang, YD,Yuan, JM,et al. Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing[J]. CEREBRAL CORTEX,2019,29(2):838-851.
APA	Yang, LX,Yang, YD,Yuan, JM,Sun, Y,Dai, JP,&Su, B.(2019).Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing.CEREBRAL CORTEX,29(2),838-851.
MLA	Yang, LX,et al."Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing".CEREBRAL CORTEX 29.2(2019):838-851.