Identification and Analysis of the Biosynthetic Gene Cluster Encoding the Thiopeptide Antibiotic Cyclothiazomycin in Streptomyces hygroscopicus 10-22

CORC > 上海有机化学研究所 > 中国科学院上海有机化学研究所 > 上海有机化学研究所

	Identification and Analysis of the Biosynthetic Gene Cluster Encoding the Thiopeptide Antibiotic Cyclothiazomycin in Streptomyces hygroscopicus 10-22
	Wang J(王绛) ; Yu Y(虞沂) ; Tang KX(唐克轩) ; Liu W(刘文) ; He XY(贺新义) ; Huang X(黄曦) ; Deng ZX(邓子新)
刊名	Appl. Environ. Microbiol.
	2010
卷号	76 期号:7 页码:2335-2344
ISSN号	0099-2240
其他题名	发现和分析在Streptomyces hygroscopicus 10-22中负责硫肽类抗生素环噻唑霉素形成的生物合成基因簇
通讯作者	贺新义 ; 黄曦
英文摘要	Thiopeptide antibiotics are an important class of natural products resulting from posttranslational modifications of ribosomally synthesized peptides. Cyclothiazomycin is a typical thiopeptide antibiotic that has a unique bridged macrocyclic structure derived from an 18-amino-acid structural peptide. Here we reported cloning, sequencing, and heterologous expression of the cyclothiazomycin biosynthetic gene cluster from Streptomyces hygroscopicus 10-22. Remarkably, successful heterologous expression of a 22.7-kb gene cluster in Streptomyces lividans 1326 suggested that there is a minimum set of 15 open reading frames that includes all of the functional genes required for cyclothiazomycin production. Six genes of these genes, cltBCDEFG flanking the structural gene cltA, were predicted to encode the enzymes required for the main framework of cyclothiazomycin, and two enzymes encoded by a putative operon, cltMN, were hypothesized to participate in the tailoring step to generate the tertiary thioether, leading to the final cyclization of the bridged macrocyclic structure. This rigorous bioinformatics analysis based on heterologous expression of cyclothiazomycin resulted in an ideal biosynthetic model for us to understand the biosynthesis of thiopeptides.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1128/AEM.01790-09
语种	英语
WOS记录号	WOS:000275844200035
公开日期	2013-08-21
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/27799]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	Wang J,Yu Y,Tang KX,et al. Identification and Analysis of the Biosynthetic Gene Cluster Encoding the Thiopeptide Antibiotic Cyclothiazomycin in Streptomyces hygroscopicus 10-22[J]. Appl. Environ. Microbiol.,2010,76(7):2335-2344.
APA	王绛.,虞沂.,唐克轩.,刘文.,贺新义.,...&邓子新.(2010).Identification and Analysis of the Biosynthetic Gene Cluster Encoding the Thiopeptide Antibiotic Cyclothiazomycin in Streptomyces hygroscopicus 10-22.Appl. Environ. Microbiol.,76(7),2335-2344.
MLA	王绛,et al."Identification and Analysis of the Biosynthetic Gene Cluster Encoding the Thiopeptide Antibiotic Cyclothiazomycin in Streptomyces hygroscopicus 10-22".Appl. Environ. Microbiol. 76.7(2010):2335-2344.