Structural insights into the recognition of phosphorylated Hop1 by Mek1

doi:10.1107/S2059798318011993

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Structural insights into the recognition of phosphorylated Hop1 by Mek1
	Xie, Changlin 1,2; He, Chao 3,4; Jiang, Yiyang 2; Yu, Hailong 2; Cheng, Lin 2; Nshogoza, Gilbert 2; Ala, Moududee Sayed 2; Tian, Changlin 1,2; Wu, Jihui 2; Shi, Yunyu 1,2
刊名	ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
	2018-10-01
卷号	74 页码:1027-1038
关键词	meiotic recombination Mek1 Hop1 FHA domain phosphointeraction
ISSN号	2059-7983
DOI	10.1107/S2059798318011993
通讯作者	Shi, Yunyu(yyshi@ustc.edu.cn) ; Li, Fudong(lifudong@ustc.edu.cn)
英文摘要	The FHA domain-containing protein Mek1 is a meiosis-specific kinase that is involved in the regulation of interhomolog recombination in meiosis in Saccharomyces cerevisiae. The recruitment and activation of Mek1 require the phosphorylation of the chromosome axis protein Hop1 at Thr318 (pT318), which is necessary for recognition by the Mek1 FHA domain. Here, crystal structures of the Mek1 FHA domain in the apo state and in complex with the Hop1 pT318 peptide are presented, demonstrating that the hydrophobic residues Phe320 and Val321 at the pT+2 and pT+3 positions in the ligand contribute to the preferential recognition. It was further found that in Schizosaccharomyces pombe Mek1 FHA binds both pT15 in its N-terminal SQ/TQ cluster domain (SCD) and pT270 in the Hop1 SCD. The results revealed the structural basis for the preferential recognition of phosphorylated Hop1 by Mek1 in S. cerevisiae and facilitate the understanding of the interaction between the S. pombe Mek1 FHA domain and its binding targets.
资助项目	Chinese National Natural Science Foundation[31330018] ; Chinese National Natural Science Foundation[31500590] ; Chinese National Natural Science Foundation[31400643] ; Ministry of Science and Technology of China[2016YFA0500700] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010101] ; Fundamental Research Funds for the Central Universities[WK2070000095]
WOS关键词	FORKHEAD-ASSOCIATED DOMAIN ; MEIOTIC RECOMBINATION INITIATION ; STRAND BREAK REPAIR ; FHA DOMAIN ; SYNAPTONEMAL COMPLEX ; YEAST MEIOSIS ; SCHIZOSACCHAROMYCES-POMBE ; LINEAR ELEMENTS ; BUDDING YEAST ; INTERHOMOLOG RECOMBINATION
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
语种	英语
出版者	INT UNION CRYSTALLOGRAPHY
WOS记录号	WOS:000446420600010
资助机构	Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/39513]
专题	合肥物质科学研究院_中科院强磁场科学中心
通讯作者	Shi, Yunyu; Li, Fudong
作者单位	1.Chinese Acad Sci, High Magnet Field Lab, 50 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, 96 Jinzhai Rd, Hefei 230026, Anhui, Peoples R China 3.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei 230601, Anhui, Peoples R China 4.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Xie, Changlin,He, Chao,Jiang, Yiyang,et al. Structural insights into the recognition of phosphorylated Hop1 by Mek1[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,2018,74:1027-1038.
APA	Xie, Changlin.,He, Chao.,Jiang, Yiyang.,Yu, Hailong.,Cheng, Lin.,...&Li, Fudong.(2018).Structural insights into the recognition of phosphorylated Hop1 by Mek1.ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,74,1027-1038.
MLA	Xie, Changlin,et al."Structural insights into the recognition of phosphorylated Hop1 by Mek1".ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 74(2018):1027-1038.