Combining an in silico approach with an animal experiment to investigate the protective effect of troxerutin for treating acute lung injury

doi:10.1186/s12906-019-2515-7

	Combining an in silico approach with an animal experiment to investigate the protective effect of troxerutin for treating acute lung injury
	Li, Ying 2; Ma, Pan 2; Fu, Jin 2; Wu, Jingjing 1; Wu, Xue 1
刊名	BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
	2019-06-10
卷号	19 页码:11
关键词	Troxerutin In silico prediction Acute lung injury Protective effect Inflammatory response
ISSN号	1472-6882
DOI	10.1186/s12906-019-2515-7
通讯作者	Li, Ying(liyingjzykd@163.com)
英文摘要	BackgroundTroxerutin (TRX), a naturally occurring flavonoid in various fruits, has been reported to exhibit numerous pharmacological and biological activities in vitro and in vivo. However, the molecular mechanisms underlying TRX as a treatment for disease are poorly understood.MethodsUsing pharmacophore mapping and inverse docking, a set of potential TRX target proteins that have been associated with multiple forms of diseases was obtained. Bioinformatic analyses were performed using the Enrichr and STRING servers to analyse the related biological processes and protein-protein networks. Furthermore, we investigated the potential protective effect of TRX against lipopolysaccharide-induced acute lung injury (ALI) using a mouse model. Morphological changes in the lungs were assessed using haematoxylin and eosin staining. Inflammatory cytokines, tumour necrosis factor- (TNF-), interleukin-1 (IL-1), IL-6 and IL-10 were investigated using ELISA. Activation of MAPK and NF-B was detected using western blotting.ResultsOur network pharmacology analysis revealed the existence of multiple TRX-related chemical-target interactions and the related biological processes. We found that pretreatment with TRX protected against histological changes and obviously regulated the inflammatory cell counts and inflammatory cytokine levels in bronchoalveolar lavage fluid. Based on bioinformatic and western blot analyses, TRX may exert a protective effect against ALI by inhibiting MAPK and NF-B signalling.ConclusionsTRX can ameliorate pulmonary injury by inhibiting the MAPK and NF-B signalling pathways and has a potential protective effect against ALI. This study may be helpful for understanding the mechanisms underlying TRX action and for discovering new drugs from plants for the treatment of ALI.
WOS关键词	NF-KAPPA-B ; ISCHEMIA/REPERFUSION INJURY ; SIGNALING PATHWAYS ; WEB SERVER ; HIGH-FAT ; INFLAMMATION ; MECHANISMS ; MOLECULES ; TARGETS ; MEMORY
WOS研究方向	Integrative & Complementary Medicine
语种	英语
出版者	BMC
WOS记录号	WOS:000470988200003
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/176139]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Li, Ying
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian, Peoples R China 2.Jinzhou Med Univ, Lab Teaching Ctr Basic Med, Jinzhou 121001, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Li, Ying,Ma, Pan,Fu, Jin,et al. Combining an in silico approach with an animal experiment to investigate the protective effect of troxerutin for treating acute lung injury[J]. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2019,19:11.
APA	Li, Ying,Ma, Pan,Fu, Jin,Wu, Jingjing,&Wu, Xue.(2019).Combining an in silico approach with an animal experiment to investigate the protective effect of troxerutin for treating acute lung injury.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,19,11.
MLA	Li, Ying,et al."Combining an in silico approach with an animal experiment to investigate the protective effect of troxerutin for treating acute lung injury".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 19(2019):11.