Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity

doi:10.1007/s12010-019-02954-x

	Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity
	Feng, Yanbin; Zhang, Yunxiu; Ding, Wei; Wu, Peichun; Cao, Xupeng; Xue, Song
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2019-07-01
卷号	188 期号:3 页码:824-835
关键词	Acyltransferase PDAT Acyl donor Biocatalyst Enzyme Acyl acceptor
ISSN号	0273-2289
DOI	10.1007/s12010-019-02954-x
通讯作者	Cao, Xupeng(c_x_p@dicp.ac.cn) ; Xue, Song(xuesong@dicp.ac.cn)
英文摘要	Triacylglycerols are considered one of the most promising feedstocks for biofuels. Phospholipid:diacylglycerol acyltransferase (PDAT), responsible for the last step of triacylglycerol synthesis in the acyl-CoA-independent pathway, has attracted much attention by catalyzing membrane lipid transformation. However, due to lack of biochemical and enzymatic studies, PDAT has not carried forward in biocatalyst application. Here, the PDAT from Saccharomyces cerevisiae was expressed in Pichia pastoris. The purified enzymes were studied using different acyl donors and acceptors by thin layer chromatography and gas chromatography. In addition of the preferred acyl donor of PE and PC, the results identified that ScPDAT was capable of using broad acyl donors such as PA, PS, PG, MGDG, DGDG, and acyl-CoA, and ScPDAT was more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, ScPDAT preferred 1,2 to 1,3-diacylglycerol (DAG), while 12:0/12:0 DAG was identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 DAG. Additionally, ScPDAT reveals esterification activity that can utilize methanol as acyl acceptor to generate fatty acid methyl esters. The results fully expand the enzymatic selectivity of ScPDAT and provide fundamental knowledge for synthesis of triacylglycerol-derived biofuels.
资助项目	National Natural Science Foundation of China[21576253] ; National Natural Science Foundation of China[21877110] ; National Natural Science Foundation of China[31470432]
WOS关键词	DIACYLGLYCEROL ACYLTRANSFERASE ; PHOSPHOLIPID/DIACYLGLYCEROL ACYLTRANSFERASE ; TRIACYLGLYCEROL ; ENZYME ; PLANT ; OIL ; DEGRADATION ; METABOLISM ; YEAST
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000471851400016
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175940]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Cao, Xupeng; Xue, Song
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Feng, Yanbin,Zhang, Yunxiu,Ding, Wei,et al. Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2019,188(3):824-835.
APA	Feng, Yanbin,Zhang, Yunxiu,Ding, Wei,Wu, Peichun,Cao, Xupeng,&Xue, Song.(2019).Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,188(3),824-835.
MLA	Feng, Yanbin,et al."Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 188.3(2019):824-835.