One-Step Preparation of Zwitterionic-Rich Hydrophilic Hydrothermal Carbonaceous Materials for Enrichment of N-Glycopeptides

doi:10.1021/acssuschemeng.9b01382

	One-Step Preparation of Zwitterionic-Rich Hydrophilic Hydrothermal Carbonaceous Materials for Enrichment of N-Glycopeptides
	Li, Xiaowei 1,3; Zhang, Haiyang 1,2; Zhang, Na 1,3; Ma, Shujuan 1,2; Ou, Junjie 1; Ye, Mingliang 1
刊名	ACS SUSTAINABLE CHEMISTRY & ENGINEERING
	2019-07-01
卷号	7 期号:13 页码:11511-11520
关键词	Glucose-derived Hydrothermal Zwitterionic hydrophilic interaction liquid chromatography ZIC-HILIC Liquid chromatography-mass spectrometry/mass spectrometry LC-MS/MS N-Glycopeptide Enrichment
ISSN号	2168-0485
DOI	10.1021/acssuschemeng.9b01382
通讯作者	Ou, Junjie(junjieou@dicp.ac.cn)
英文摘要	Two kinds of novel zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) materials decorated with phosphorylcholine or sulfobetaine type zwitterionic groups were facilely synthesized employing hydrothermal carbonization (HTC) reaction, in which sustainable carbohydrate (glucose) could be converted into functionalized carbonaceous materials with strong hydrophilicity using a one-step and environmentally mild process. The properties of obtained materials were characterized by helium ion microscopy (HIM), N-2 adsorption/desorption measurement, static water contact angle measurement, elemental analysis, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and zeta-potential. As a result, 26 N-glycopeptides with S/N above 20 could be detected from 150 fmol IgG digests after hydrophilic interaction chromatography (HILIC) enrichment by using as-synthesized material HTC-Glc-10%MPC. The limit of detection in matrix assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF MS) analysis was as low as 5 fmol and enrichment selectivity reached 100:1. Furthermore, 175 N-glycosylation sites in 285 N-glycopeptides from 92 glycoproteins were identified from 2 mu L of human serum. This work provided a new, simple, and green way to synthesize an N-glycopeptide enrichment material for liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis of glycoproteomes.
资助项目	China State Key Basic Research Program[2017YEA0505004] ; China State Key Basic Research Program[2016YFA0501402] ; National Natural Sciences Foundation of China[21575141] ; CAS-Weigao Research & Development Program[[2017]-009]
WOS关键词	SOLID-PHASE EXTRACTION ; HIGHLY EFFICIENT ENRICHMENT ; METAL-ORGANIC FRAMEWORKS ; SELECTIVE ENRICHMENT ; LINKED GLYCOPEPTIDES ; MESOPOROUS SILICA ; MAGNETIC NANOPARTICLES ; STATIONARY-PHASE ; FACILE SYNTHESIS ; L-CYSTEINE
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Engineering
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000474474800052
资助机构	China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175513]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Ou, Junjie
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Northwest Univ, Coll Chem & Mat Sci, Minist Educ, Key Lab Synthet & Nat Funct Mol Chem, Xian 710069, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaowei,Zhang, Haiyang,Zhang, Na,et al. One-Step Preparation of Zwitterionic-Rich Hydrophilic Hydrothermal Carbonaceous Materials for Enrichment of N-Glycopeptides[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2019,7(13):11511-11520.
APA	Li, Xiaowei,Zhang, Haiyang,Zhang, Na,Ma, Shujuan,Ou, Junjie,&Ye, Mingliang.(2019).One-Step Preparation of Zwitterionic-Rich Hydrophilic Hydrothermal Carbonaceous Materials for Enrichment of N-Glycopeptides.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,7(13),11511-11520.
MLA	Li, Xiaowei,et al."One-Step Preparation of Zwitterionic-Rich Hydrophilic Hydrothermal Carbonaceous Materials for Enrichment of N-Glycopeptides".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 7.13(2019):11511-11520.