RNA interference in the oleaginous yeast Rhodosporidium toruloides

doi:10.1093/femsyr/foz031

	RNA interference in the oleaginous yeast Rhodosporidium toruloides
	Liu, Xiangjian 1,2; Zhang, Yue 2,3; Liu, Hongdi 2; Jiao, Xiang 2; Zhang, Qi 2; Zhang, Sufang 2; Zhao, Zongbao Kent 2
刊名	FEMS YEAST RESEARCH
	2019-05-01
卷号	19 期号:3 页码:10
关键词	Rhodosporidium toruloides RNAi endoribonuclease Dicer ATG8 FAS1 FAS2
ISSN号	1567-1356
DOI	10.1093/femsyr/foz031
通讯作者	Zhang, Sufang(zsfjxy@dicp.ac.cn)
英文摘要	The red yeast Rhodosporidium toruloides is an excellent microbial host for production of carotenoids, neutral lipids and valuable enzymes. In recent years, genetic tools for gene expression and gene disruption have been developed for this red yeast. However, methods remain limited in terms of fine-tuning gene expression. In this study, we first demonstrated successful implementation of RNA interference (RNAi) in R. toruloides NP11, which was applied to down-regulate the expression of autophagy related gene 8 (ATG8), and fatty acid synthase genes (FAS1 and FAS2), respectively. Compared with the control strain, RNAi-engineered strains showed a silencing efficiency ranging from 11% to 92%. The RNAi approach described here ensures selective inhibition of the target gene expression, and should expand our capacity in the genetic manipulation of R. toruloides for both fundamental research and advanced cell factory development.
资助项目	National Natural Science Foundation of China[51761145014] ; Dalian Institute of Chemical Physics, CAS[DICPQIBEBT UN201706] ; Dalian Institute of Chemical Physics, CAS[DMTO201701]
WOS关键词	DOUBLE-STRANDED-RNA ; GENE-EXPRESSION ; TRANSFORMATION ; RHODOTORULA ; SYSTEM ; GENOME ; CAROTENOIDS ; HYDROLYSATE ; REPRESSION ; PROMOTERS
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology ; Mycology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000474809700019
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian Institute of Chemical Physics, CAS ; Dalian Institute of Chemical Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian Institute of Chemical Physics, CAS ; Dalian Institute of Chemical Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian Institute of Chemical Physics, CAS ; Dalian Institute of Chemical Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian Institute of Chemical Physics, CAS ; Dalian Institute of Chemical Physics, CAS
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175443]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Sufang
作者单位	1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiangjian,Zhang, Yue,Liu, Hongdi,et al. RNA interference in the oleaginous yeast Rhodosporidium toruloides[J]. FEMS YEAST RESEARCH,2019,19(3):10.
APA	Liu, Xiangjian.,Zhang, Yue.,Liu, Hongdi.,Jiao, Xiang.,Zhang, Qi.,...&Zhao, Zongbao Kent.(2019).RNA interference in the oleaginous yeast Rhodosporidium toruloides.FEMS YEAST RESEARCH,19(3),10.
MLA	Liu, Xiangjian,et al."RNA interference in the oleaginous yeast Rhodosporidium toruloides".FEMS YEAST RESEARCH 19.3(2019):10.