Structural Analysis of High Degree Polymerization Isomers of Human Milk Oligosaccharides Based on Electrospray Ionization-Quadrupole-Time of Flight Mass Spectrometry

doi:10.19756/j.issn.0253-3820.191167

	Structural Analysis of High Degree Polymerization Isomers of Human Milk Oligosaccharides Based on Electrospray Ionization-Quadrupole-Time of Flight Mass Spectrometry
	Li Xin 1,2; Li Jia-Qi 1; Jin Gao-Wa 1; Yu Long 1; Guo Zhi-Mou 1; Liang Xin-Miao 1; Liu Shuai 2; Yan Jing-Yu 1
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2019-07-01
卷号	47 期号:7 页码:1106-1113
关键词	Human milk oligosaccharides Isomers Structural analysis Electrospray ionization-quadrupole-time of flight mass spectrometry
ISSN号	0253-3820
DOI	10.19756/j.issn.0253-3820.191167
通讯作者	Liu Shuai(liushuai_129@163.com) ; Yan Jing-Yu(yanjingyu@dicp.ac.cn)
英文摘要	Human milk oligosaccharides are a kind of natural prebiotics that exist in human milk. They play an irreplaceable role in the growth of infantile intestinal flora, the improvement of immune system and the resistance to pathogen infection. The function of human milk oligosaccharides is closely related to their structures which are complex with various linkages. Besides , the oligosaccharides have a large number of isomers , the number and complexity of which increase dramatically with the degrees of their polymerization. Therefore , the structural analysis of oligosaccharide isomers with a high degree of polymerization is one of the difficulties in the study of human milk oligosaccharides , and is of great significance for the study of the biological function of oligosaccharides. In this study , electrospray ionization-quadrupole-time of flight mass spectrometry ( ESI-Q/TOF-MS ) was used to analyze the structures of eighteen high-degree polymerized human milk oligosaccharides in a negative ion mode , and three of them were firstly reported. Differences of fragment ions between the isomers were discussed, and the cleavage rules of oligosaccharide isomers were summarized. This study provided a scientific basis for the structural analysis of the complex isomers of highly polymerized human milk oligosaccharides and the discovery of novel sugar structures.
资助项目	National Key Research and Development Program of China[2017YFD0400601,2016YFB0301502] ; National Natural Science Foundation of China[U1608255]
WOS关键词	ANTIGENS ; NEEDS
WOS研究方向	Chemistry
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000473647100021
资助机构	National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175406]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu Shuai; Yan Jing-Yu
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Med Univ, Dept Biochem & Mol Biol, Dalian 116044, Peoples R China
推荐引用方式 GB/T 7714	Li Xin,Li Jia-Qi,Jin Gao-Wa,et al. Structural Analysis of High Degree Polymerization Isomers of Human Milk Oligosaccharides Based on Electrospray Ionization-Quadrupole-Time of Flight Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2019,47(7):1106-1113.
APA	Li Xin.,Li Jia-Qi.,Jin Gao-Wa.,Yu Long.,Guo Zhi-Mou.,...&Yan Jing-Yu.(2019).Structural Analysis of High Degree Polymerization Isomers of Human Milk Oligosaccharides Based on Electrospray Ionization-Quadrupole-Time of Flight Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,47(7),1106-1113.
MLA	Li Xin,et al."Structural Analysis of High Degree Polymerization Isomers of Human Milk Oligosaccharides Based on Electrospray Ionization-Quadrupole-Time of Flight Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 47.7(2019):1106-1113.