enhancedmultiphosphopeptideenrichmentandnanolcesiqtofmsdetectionstrategyusingclickoegcdmatrix | |
Zhao Yanyan1; Wang Liheng2; Guo Zhimou2; Chi Xiaofei2; Ma Xiaochi1; Qi Yan1; Fang Shinong1; Li Xiuling2; Liang Xinmiao2 | |
刊名 | chemicalresearchinchineseuniversities |
2015-01-01 | |
卷号 | 000期号:001页码:44 |
ISSN号 | 1005-9040 |
英文摘要 | Detection and determination of multi-phosphopeptides from protein digestion products are difficult due to the low abundance and ion-suppression effect arised from the existence of their high-abundance counterparts. Click OEG-CDolio(ethylene glycol)(OEG) linked β-cyclodextrin(CD) matrix has shown excellent performance in phosphopeptide enrichment. However, few multi-phosphopeptides were detected previously. In this investigation, an improved method aiming at enhancing the enrichment selectivity and mass spectrometry(MS) detection of multi-phosphopeptide was developed via optimizing the sample loading amount on per mg of matrix. Mass spectra were obtained on a Nano liquid chromatography-electrospray ionization-quadrupole time of flight-mass spectrometry (LC-ESI-qTOF-MS). The enrichment selectivity of double-phosphopeptide could be enhanced with the increase of loading amount on per mg of matrix when taking the mixture of mono-, double-, and non-phosphopeptides as probe. Furthermore, the multi-phosphopeptide enrichment selectivity was enhanced under the condition of optimized loading amount when taking the product of typtic digestion a-casein as test sample. When the loading amount was 573 pmol/mg matrix, up to 20 a-casein phosphopeptide signals(including 15 multi-phosphopeptides) were detected. The result is much better than that of our previous report. The reduction of ion-suppression effect arised from the existence of high-abundance non- or mono-phosphopeptides and the stronger interactions between multiphosphopepides and the matrix were contributed to the result. The study could be helpful to the better utilization of Click OEG-CD matrix in the enrichment of multi-phosphopeptide from complex biosamples in the subsequent investigation. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/174973] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.大连医科大学 2.中国科学院大连化学物理研究所 |
推荐引用方式 GB/T 7714 | Zhao Yanyan,Wang Liheng,Guo Zhimou,et al. enhancedmultiphosphopeptideenrichmentandnanolcesiqtofmsdetectionstrategyusingclickoegcdmatrix[J]. chemicalresearchinchineseuniversities,2015,000(001):44. |
APA | Zhao Yanyan.,Wang Liheng.,Guo Zhimou.,Chi Xiaofei.,Ma Xiaochi.,...&Liang Xinmiao.(2015).enhancedmultiphosphopeptideenrichmentandnanolcesiqtofmsdetectionstrategyusingclickoegcdmatrix.chemicalresearchinchineseuniversities,000(001),44. |
MLA | Zhao Yanyan,et al."enhancedmultiphosphopeptideenrichmentandnanolcesiqtofmsdetectionstrategyusingclickoegcdmatrix".chemicalresearchinchineseuniversities 000.001(2015):44. |
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