Advances in exosome isolation methods and their applications in proteomic analysis of biological samples

doi:10.1007/s00216-019-01982-0

	Advances in exosome isolation methods and their applications in proteomic analysis of biological samples
	Hou, Rui 1; Li, Yilan 2; Sui, Zhigang 1; Yuan, Huiming 1; Yang, Kaiguang 1; Liang, Zhen 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2019-08-01
卷号	411 期号:21 页码:5351-5361
关键词	Exosomes Extracellular vesicles Isolation Proteomic analysis
ISSN号	1618-2642
DOI	10.1007/s00216-019-01982-0
通讯作者	Yuan, Huiming(huimingyuan@dicp.ac.cn) ; Zhang, Lihua(lihuazhang@dicp.ac.cn)
英文摘要	Exosomes are membrane-bound vesicles secreted by cells, and contain various important biological molecules, such as lipids, proteins, messenger RNAs, microRNAs, and noncoding RNAs. Emerging evidence demonstrates that proteomic analysis of exosomes is of great significance in studying metabolic diseases, tumor metastasis, immune regulation, and so forth. However, exosome proteomic analysis has high requirements with regard to the purity of collected exosomes. Here recent advances in the methods for isolating exosomes and their applications in proteomic analysis are summarized.
资助项目	National Key R&D Program of China[2017YFA0505003] ; National Natural Science Foundation of China[21775149] ; National Natural Science Foundation of China[21725506] ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences[DICP TMSR201601]
WOS关键词	CELL-CULTURE SUPERNATANT ; EXTRACELLULAR VESICLES ; LIQUID-CHROMATOGRAPHY ; RAPID ISOLATION ; PLASMA ; BLOOD ; ULTRACENTRIFUGATION ; ENRICHMENT ; DELIVERY ; QUANTIFICATION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000479055200002
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/173570]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yuan, Huiming; Zhang, Lihua
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hou, Rui,Li, Yilan,Sui, Zhigang,et al. Advances in exosome isolation methods and their applications in proteomic analysis of biological samples[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(21):5351-5361.
APA	Hou, Rui.,Li, Yilan.,Sui, Zhigang.,Yuan, Huiming.,Yang, Kaiguang.,...&Zhang, Yukui.(2019).Advances in exosome isolation methods and their applications in proteomic analysis of biological samples.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(21),5351-5361.
MLA	Hou, Rui,et al."Advances in exosome isolation methods and their applications in proteomic analysis of biological samples".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.21(2019):5351-5361.