Microfluidic strategies for label-free exosomes isolation and analysis

doi:10.1016/j.trac.2019.06.037

	Microfluidic strategies for label-free exosomes isolation and analysis
	Su, Wentao 1,2; Li, Hongjing 5; Chen, Wenwen 1,2; Qin, Jianhua 1,2,3,4
刊名	TRAC-TRENDS IN ANALYTICAL CHEMISTRY
	2019-09-01
卷号	118 页码:686-698
关键词	Label-free Microfluidics Exosome isolation Disease diagnostics On-chip analysis
ISSN号	0165-9936
DOI	10.1016/j.trac.2019.06.037
通讯作者	Qin, Jianhua(jhqin@dicp.ac.cn)
英文摘要	Exosomes are nanoscale membrane vesicles (similar to 30-150 nm) actively released by most types of cells, which has great potential for non-invasive disease diagnostics. Current isolation techniques such as ultracentrifugation face a number of drawbacks including low integrity, low purity and long processing times. This represents an interesting challenge for microfluidic technologies, from integrity perspective as well as for enhanced purity capabilities, point-of-care acquisition and diagnosis. In this review, we highlight the label-free microfluidic advances for isolation and analysis of exosomes based on their size, deformability and electrical characteristics. These new capabilities are expected to advance fundamental research while paving the way for routine exosome-based personalized medicine. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	National Key R&D Program of China[2017YFB0405404] ; Natural Science Foundation of China[21607151] ; Natural Science Foundation of China[81573394] ; Natural Science Foundation of China[31671038] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16020900] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB29050301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDBS01030200] ; Innovation Program of Science and Research from the DICP, CAS[DICP TMSR201601] ; Innovation Program of Science and Research from the DICP, CAS[DICP I201934]
WOS关键词	CONTINUOUS PARTICLE SEPARATION ; FIELD-FLOW FRACTIONATION ; EXTRACELLULAR VESICLES ; LIQUID BIOPSY ; BLOOD ; MICROVESICLES ; CANCER ; CHIP ; POPULATIONS ; DEVICE
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000486383900051
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Key R&D Program of China ; National Key R&D Program of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Key R&D Program of China ; National Key R&D Program of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Key R&D Program of China ; National Key R&D Program of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/172645]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Qin, Jianhua
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Div Biotechnol, Dalian, Peoples R China 3.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China 4.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China 5.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Su, Wentao,Li, Hongjing,Chen, Wenwen,et al. Microfluidic strategies for label-free exosomes isolation and analysis[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2019,118:686-698.
APA	Su, Wentao,Li, Hongjing,Chen, Wenwen,&Qin, Jianhua.(2019).Microfluidic strategies for label-free exosomes isolation and analysis.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,118,686-698.
MLA	Su, Wentao,et al."Microfluidic strategies for label-free exosomes isolation and analysis".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 118(2019):686-698.