Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol
	Zhang, Weiwei 1; Niu, Zongliang 1; Liao, Chunyang1 ; Chen, Lingxin1,2
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2013-05-01
卷号	97 期号:9 页码:4095-4104
关键词	Diethylstilbestrol Pseudomonas sp. Degradation Metabolite Cytotoxicity
ISSN号	0175-7598
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. lxchen@yic.ac.cn
产权排序	[Zhang, Weiwei; Niu, Zongliang; Liao, Chunyang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
中文摘要	Since diethylstilbestrol (DES) interrupts endocrine systems and generates reproductive abnormalities in both wildlife and human beings, methods to remove DES from the environments are urgently recommended. In this study, bacterial strain J51 was isolated and tested to effectively degrade DES. J51 was identified as Pseudomonas sp. based on its nucleotide sequence of 16S rRNA. The quinoprotein alcohol dehydrogenase and isocitrate lyase were identified to be involved in DES degradation by MALDI-TOF-TOF MS/MS analysis. In the presence of 40 mg/l DES, increase of the genes encoding quinoprotein alcohol dehydrogenase and isocitrate lyase in both RNA and protein levels was determined. The HPLC/MS analysis showed that DES was hydrolyzed to a major degrading metabolite DES-4-semiquinone. It was the first time to demonstrate the characteristics of DES degradation by specific bacterial strain and the higher degradation efficiency indicated the potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments.
英文摘要	Since diethylstilbestrol (DES) interrupts endocrine systems and generates reproductive abnormalities in both wildlife and human beings, methods to remove DES from the environments are urgently recommended. In this study, bacterial strain J51 was isolated and tested to effectively degrade DES. J51 was identified as Pseudomonas sp. based on its nucleotide sequence of 16S rRNA. The quinoprotein alcohol dehydrogenase and isocitrate lyase were identified to be involved in DES degradation by MALDI-TOF-TOF MS/MS analysis. In the presence of 40 mg/l DES, increase of the genes encoding quinoprotein alcohol dehydrogenase and isocitrate lyase in both RNA and protein levels was determined. The HPLC/MS analysis showed that DES was hydrolyzed to a major degrading metabolite DES-4-semiquinone. It was the first time to demonstrate the characteristics of DES degradation by specific bacterial strain and the higher degradation efficiency indicated the potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments.
学科主题	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	BISPHENOL-A ; BIOREMEDIATION ; CHEMICALS ; BACTERIA
收录类别	SCI
资助信息	Innovation Projects of the Chinese Academy of Sciences [KZCX2-EW-206]; National Natural Science Foundation of China (NSFC) [31200041, 20975089, 20907039]; Doctoral Foundation of Shandong Province [BS2011SW056]; Department of Science and Technology of Yantai City of China [2010235]; 100 Talents Program of the Chinese Academy of Sciences
原文出处	10.1007/s00253-012-4648-1
语种	英语
WOS记录号	WOS:000317681800031
公开日期	2013-08-15
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6625]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_污染过程与控制实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Weiwei,Niu, Zongliang,Liao, Chunyang,et al. Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(9):4095-4104.
APA	Zhang, Weiwei,Niu, Zongliang,Liao, Chunyang,&Chen, Lingxin.(2013).Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(9),4095-4104.
MLA	Zhang, Weiwei,et al."Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.9(2013):4095-4104.