Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis

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	Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis
	Zhao QF(赵群飞) ; He QL(贺庆利) ; Ding W(丁伟) ; Tang MC(唐满成) ; Kang QJ(康前进) ; Yu Y(虞沂) ; Deng W(邓玮) ; Zhang Q(张琪) ; Fang J(方洁) ; Tang GL(唐功利) ; Liu W(刘文)
刊名	Chem. Biol.
	2008
卷号	15 期号:7 页码:693-705
ISSN号	1074-5521
其他题名	Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis
通讯作者	刘文
英文摘要	Azinomycin B is a complex natural product containing densely assembled functionalities with potent antitumor activity. Cloning and sequence analysis of the azi gene cluster revealed an iterative type I polyketide synthase (PKS) gene, five nonribosomal peptide synthetases (NRPSs) genes and numerous genes encoding the biosynthesis of unusual building blocks and tailoring steps for azinomycin B production. Characterization of AziB as a 5-methyl-naphthoic acid (NP) synthase showed a distinct selective reduction pattern in aromatic polyketide biosynthesis governed by bacterial iterative type I PKSs. Heterologous expression established the PKS-post modification route from 5-methyl-NPA to reach the first building block 3-methoxy-5-methyl-NPA. This proposed azinomycin B biosynthetic pathway sets the stage to investigate the enzymatic mechanisms for building structurally unique and pharmaceutically important groups, including the unprecedented azabicyclic ring system and highly active epoxide moiety.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.chembiol.2008.05.021
语种	英语
WOS记录号	WOS:000258019700010
公开日期	2013-08-14
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/27713]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	Zhao QF,He QL,Ding W,et al. Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis[J]. Chem. Biol.,2008,15(7):693-705.
APA	赵群飞.,贺庆利.,丁伟.,唐满成.,康前进.,...&刘文.(2008).Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis.Chem. Biol.,15(7),693-705.
MLA	赵群飞,et al."Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis".Chem. Biol. 15.7(2008):693-705.