A Proteomic Study on Cell Cycle Progression of Endothelium Exposed to Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E

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	A Proteomic Study on Cell Cycle Progression of Endothelium Exposed to Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E
	Li AL; Li HW; Jin G(靳刚); Xiu RJ
	2003-02-20
会议名称	5th Asian Congress for Microcirculation, FEB 20-22, 2003 MANILA, PHILIPPINES
通讯作者	靳刚
中文摘要	This study was designed to comprehensively analyze the differential expression of proteins from human umbilical vein endothelial cells (HUVECs) exposed to tumor conditioned medium (TCM) and to identify the key regulator in the cell cycle progression. The HUVECs were exposed to TCM from breast carcinoma cell line MDA-MB-231, then their cell cycle distribution was measured by flow cytometer (FCM). The role of protein in cell cycle progression was detected via two-dimensional polyacrylamide gel electrophoresis (2-DE) and western blotting. Following the stimulation of TCM, HUVECs showed a more cells in the S phase than did the negative control group (ECGF-free medium with 20% FBS), but the HUVECs' level was similar to the positive control group (medium with 25 mug/ml ECGF and 20% FBS). Increased expression of cyclin D-1/E and some changes in other related proteins occurred after incubation with TCM. From our results, we can conclude that breast carcinoma cell line MDA-MB-231 may secrete soluble pro-angiogenic factors that induce the HUVEC angiogenic switch, during which the expression of cell cycle regulator cyclin D-1/E increases and related proteins play an important role in this process.
收录类别	CPCI-S
会议录	CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2003,29(3-4):383-390
学科主题	力学
语种	英语
WOS记录号	WOS:000188240800032
内容类型	会议论文
源URL	[http://dspace.imech.ac.cn/handle/311007/13746]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Li AL,Li HW,Jin G,et al. A Proteomic Study on Cell Cycle Progression of Endothelium Exposed to Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E[C]. 见:5th Asian Congress for Microcirculation, FEB 20-22, 2003 MANILA, PHILIPPINES.