PROTEOME ANALYSIS OF ACTIN FILAMENT-ASSOCIATED PROTEINS IN THE POSTNATAL RAT CEREBELLUM

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	PROTEOME ANALYSIS OF ACTIN FILAMENT-ASSOCIATED PROTEINS IN THE POSTNATAL RAT CEREBELLUM
	Shi, N ; Tian, C ; Liang, X ; Jiang, P ; Liang, L ; Zhou, L ; Shu, Y ; Chen, P ; Wang, Y
刊名	NEUROSCIENCE
	2012
卷号	227 页码:90-101
关键词	POSTSYNAPTIC DENSITY FRACTION TANDEM MASS-SPECTROMETRY BINDING PROTEINS IDENTIFICATION CYTOSKELETON CHROMATOGRAPHY MORPHOGENESIS EXPRESSION FOREBRAIN MEMBRANE
ISSN号	0306-4522
通讯作者	Chen, P (reprint author), Hunan Normal Univ, Coll Life Sci, Key Lab Prot Chem & Dev Biol, Minist Educ, Changsha 410081, Hunan, Peoples R China.,chenp@hunnu.edu.cn ; wangying910000@yahoo.com
英文摘要	The cerebellum contains more neurons than all other brain regions combined and these cells exhibit complex circuit development and dendritic elaboration during the postnatal period. Neural development, cellular morphogenesis, and synaptic plasticity are dependent on the dynamic regulation of the actin cytoskeleton by actin-binding proteins. The identification of the actin filament interactome, including proteins developmentally regulated in the postnatal cerebellum, could help define important regulators of actin cytoskeletal dynamics in developing cerebellar neurons. Affinity purification of cerebellar proteins on F-actin columns, combined with mass spectrometry, in total, 434 actin filament-associated proteins in postnatal rat cerebellum (P7) were identified. Furthermore, semi-quantitative RT-PCR was performed to screening postnatal developmentally regulated genes involved in actin dynamics and membrane trafficking in rat cerebellum (P0-P56). As the result, nine genes encoding members of the cerebellar F-actin interactome were developmentally regulated in the transcriptional level and at least five of them exhibited a similar pattern at the protein expression level by Western blot analysis. Further fluorescent immunohistochemical observations demonstrated that the actin-associated proteins Lethal(2) giant larvae protein homolog 1 (LLGL1) and metastasis suppressor 1 (MTSS1) were specifically upregulated in granule neurons and Purkinje cells during morphogenesis of axons and dendrites. This work defines a provisional actin filament interactome in rat postnatal cerebellum and identifies several candidate proteins that may be involved in the postnatal development of the cerebellum. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.
学科主题	Neurosciences & Neurology
收录类别	SCI
资助信息	National Natural Science Foundation of China [30800551, 81070353]; Key Project of Chinese Ministry of Education [209090]; Scientific Research Foundation for the Returned Overseas Chinese Scholars; State Education Ministry, Scientific Research Fund of Hunan Provincial Education Department [08B047]; Natural Science Foundation of Hunan Province [09JJ4019]; Doctoral research initiation program; Hunan Normal University
语种	英语
公开日期	2013-06-04
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2495]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Shi, N,Tian, C,Liang, X,et al. PROTEOME ANALYSIS OF ACTIN FILAMENT-ASSOCIATED PROTEINS IN THE POSTNATAL RAT CEREBELLUM[J]. NEUROSCIENCE,2012,227:90-101.
APA	Shi, N.,Tian, C.,Liang, X.,Jiang, P.,Liang, L.,...&Wang, Y.(2012).PROTEOME ANALYSIS OF ACTIN FILAMENT-ASSOCIATED PROTEINS IN THE POSTNATAL RAT CEREBELLUM.NEUROSCIENCE,227,90-101.
MLA	Shi, N,et al."PROTEOME ANALYSIS OF ACTIN FILAMENT-ASSOCIATED PROTEINS IN THE POSTNATAL RAT CEREBELLUM".NEUROSCIENCE 227(2012):90-101.