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	A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE)
	Chao K. F. ; Zhang Y. L. ; Kong X. G. ; Zeng Q. H. ; Liu R. L. ; Wang X. ; Feng L. Y. ; Sun Y. J.
	2006
会议名称	ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005
会议地点	Changchun, China
关键词	The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore red shift of the FITC fluorescence dye emission peak was observed on different fiber surface exhibiting different interaction between FITC- labeled antibody and surface. Finally under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.
收录类别	EI
内容类型	会议论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/33668]
专题	长春光学精密机械与物理研究所_中科院长春光机所知识产出_会议论文
推荐引用方式 GB/T 7714	Chao K. F.,Zhang Y. L.,Kong X. G.,et al. A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE)[C]. 见:ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005. Changchun, China.

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