Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria
	Shi Fei 1,3; Li Nan1,2,3 ; Liu ShaoFang 3,4; Qin Song1
刊名	CHINESE SCIENCE BULLETIN
	2012-09-01
卷号	57 期号:25 页码:3270-3275
关键词	water bloom phytochrome Microcystis aeruginosa positive selection cyanobacteria spectral characteristic
ISSN号	1001-6538
通讯作者	Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.,sqin@yic.ac.cn
产权排序	[Shi Fei; Li Nan; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li Nan] Korean Ocean Res & Dev Inst, Marine Biotechnol Res Ctr, Ansan 425600, South Korea; [Shi Fei; Li Nan; Liu ShaoFang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Liu ShaoFang] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
中文摘要	The phytochrome gene from Microcystis aeruginosa FACHB-912, isolated from algal blooms in Taihu Lake in China, was sequenced and found to code for a wavelength transition light receptor protein. The gene was composed of five distinct domains: a PAS fold domain, a GAF domain, a phytochrome domain, a His Kinase A (phosphoacceptor) domain, and a histidine kinase domain. Red-absorbing phytochrome, far-red-absorbing phytochrome, and photoconversion kinetics were monitored via the spectral characteristics of the protein. To detect positive selection, homologous sequences from cyanobacterium phytochrome genes were obtained from GenBank. Potential selective pressure was identified by phylogenetic analysis with maximum likelihood analyses. The frequency of the phytochrome gene being subjected to selective constraints suggests that positive selection is a potentially important mechanism that promotes the evolution of the cyanobacteria phytochrome gene. The sites identified in this study provide targets for further research on the structural-functional role of these residues, and on the correlation with the mechanism of algal blooms.
英文摘要	The phytochrome gene from Microcystis aeruginosa FACHB-912, isolated from algal blooms in Taihu Lake in China, was sequenced and found to code for a wavelength transition light receptor protein. The gene was composed of five distinct domains: a PAS fold domain, a GAF domain, a phytochrome domain, a His Kinase A (phosphoacceptor) domain, and a histidine kinase domain. Red-absorbing phytochrome, far-red-absorbing phytochrome, and photoconversion kinetics were monitored via the spectral characteristics of the protein. To detect positive selection, homologous sequences from cyanobacterium phytochrome genes were obtained from GenBank. Potential selective pressure was identified by phylogenetic analysis with maximum likelihood analyses. The frequency of the phytochrome gene being subjected to selective constraints suggests that positive selection is a potentially important mechanism that promotes the evolution of the cyanobacteria phytochrome gene. The sites identified in this study provide targets for further research on the structural-functional role of these residues, and on the correlation with the mechanism of algal blooms.
学科主题	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	CODON-SUBSTITUTION MODELS ; ASPEN POPULUS-TREMULA ; AMINO-ACID SITES ; PHYLOGENETIC ANALYSIS ; MOLECULAR EVOLUTION ; ADAPTIVE EVOLUTION ; HISTIDINE KINASES ; NATURAL VARIATION ; LIGHT ; BACTERIOPHYTOCHROMES
收录类别	SCI
资助信息	CAS/SAFEA International Partnership Program for Creative Research Teams; Chinese Marine Microbial Center[KSCX2-YW-G-073]
语种	英语
WOS记录号	WOS:000307750500004
公开日期	2013-03-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6139]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Korean Ocean Res & Dev Inst, Marine Biotechnol Res Ctr, Ansan 425600, South Korea 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Shi Fei,Li Nan,Liu ShaoFang,et al. Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3270-3275.
APA	Shi Fei,Li Nan,Liu ShaoFang,&Qin Song.(2012).Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria.CHINESE SCIENCE BULLETIN,57(25),3270-3275.
MLA	Shi Fei,et al."Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria".CHINESE SCIENCE BULLETIN 57.25(2012):3270-3275.