Nuclear transplantation with early-embryonic cells of transgenic fish

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	Nuclear transplantation with early-embryonic cells of transgenic fish
	Chen, SP; Zhao, HB; Sun, YH; Zhang, FY; Zhu, ZY
刊名	PROGRESS IN NATURAL SCIENCE
	2000-12-01
卷号	10 期号:12 页码:925-930
关键词	fish gene transfer nuclear transplantation
ISSN号	1002-0071
通讯作者	Chen, SP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Like other transgenic animals, transgenic fishes produced by microinjection are transgenic mosaics. In order to produce homogenous transgenic fish, the transgenic blastula or gastrula cells were dissociated from Carassius auratus, Pengze var, and Cyprinus carpio, Huanghe var., and the nuclei were transferred into the mature eggs of the same species via microinjection or electro-fusion. Five nuclear-transferred Carassius auratus, Pengze var. and one Cyprinus carpio, Huanghe var. were obtained and the existence of the transgene was detected. The possibility of generating homogenous strain of transgenic fish by nuclear transplantation with transgenic early-embryonic cells is discussed.
英文摘要	Like other transgenic animals, transgenic fishes produced by microinjection are transgenic mosaics. In order to produce homogenous transgenic fish, the transgenic blastula or gastrula cells were dissociated from Carassius auratus, Pengze var, and Cyprinus carpio, Huanghe var., and the nuclei were transferred into the mature eggs of the same species via microinjection or electro-fusion. Five nuclear-transferred Carassius auratus, Pengze var. and one Cyprinus carpio, Huanghe var. were obtained and the existence of the transgene was detected. The possibility of generating homogenous strain of transgenic fish by nuclear transplantation with transgenic early-embryonic cells is discussed.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]	Materials Science ; Science & Technology - Other Topics
关键词[WOS]	ORYZIAS-LATIPES ; ESTABLISHMENT ; CULTURES ; GROWTH ; MEDAKA ; EGGS
收录类别	SCI
语种	英语
WOS记录号	WOS:000165659900006
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/10056]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Chen, SP,Zhao, HB,Sun, YH,et al. Nuclear transplantation with early-embryonic cells of transgenic fish[J]. PROGRESS IN NATURAL SCIENCE,2000,10(12):925-930.
APA	Chen, SP,Zhao, HB,Sun, YH,Zhang, FY,&Zhu, ZY.(2000).Nuclear transplantation with early-embryonic cells of transgenic fish.PROGRESS IN NATURAL SCIENCE,10(12),925-930.
MLA	Chen, SP,et al."Nuclear transplantation with early-embryonic cells of transgenic fish".PROGRESS IN NATURAL SCIENCE 10.12(2000):925-930.