Interactions of New Synthesized Fluorescent Cationic Amphiphiles Bearing Pyrene Hydrophobe with Plasmid DNA: Binding Affinities, Aggregation and Intracellular Uptake

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	Interactions of New Synthesized Fluorescent Cationic Amphiphiles Bearing Pyrene Hydrophobe with Plasmid DNA: Binding Affinities, Aggregation and Intracellular Uptake
	Sheng RL(盛瑞隆) ; Luo T(罗挺) ; Zhu YD(朱英丹) ; Li H(李慧) ; Cao AM(曹阿民)
刊名	Macromol. Biosci.
	2010
卷号	10 期号:8 页码:974-982
ISSN号	1616-5187
其他题名	带有疏水性芘的新型荧光两亲分子与质粒DNA的相互作用: 结合力、聚集以及细胞内吞
通讯作者	曹阿民
英文摘要	In this work, we prepared a novel series of cationic amphiphiles denoted as the Py-cations (Py-Gly, Py-Ala, Py-Cap, Py-G(1)-Lys and Py-G(2)-Lys) bearing fluorescent pyrene and various hydrocarbon linkers between the pyrene hydrophobe and cationic block. Employing these new cationic amphiphiles with pyrene as the fluorescent probe, the interactions between these Py-cations and plasmid DNA (pDNA) in distilled water and 0.1 M PBS buffer solution have been explored by means of UV-vis and fluorescent spectrometers, and ethidium bromide dye displacement and agarose-gel retardant assays were also implemented to evaluate their pDNA binding affinities in aqueous solution. Furthermore, the average sizes and morphologies of self-assembled Py-cation/pDNA lipoplex aggregates were examined by dynamic laser light scattering (DLS) and atomic force microscopy (AFM). It was found that these fluorescent cationic amphiphiles showed blue fluorescence emission of pyrene probe at lambda = 340 nm in distilled water while their interactions with pDNA led to new strong green emission at lambda = 490 nm, and this may be due to the stacking of pyrene and new formation of excimers via the rigid pDNA templated self-assembly. It was also revealed that the binding between new Py-cations and pDNA in aqueous solution was strongly influenced by the Py-cation hydrophobicity, charges of the cation and the presence of electrolytes. With respect to the Py-cation/pDNA aggregate morphologies, very interesting 1-D hybrid nanofibers were predominantly observed by AFM for the Py-Cap/pDNA aggregates. In addition, utilizing a COS-7 cell-line, in-vitro cellular uptakes of new cationic amphiphiles with pyrene probe were studied and visualized by fluorescent microscopy. As a result, this may provide a new approach to investigate the interactions between synthetic cationic lipids and nucleic acids, and pave an alternative clue to design new organic gene delivery carriers.
学科主题	高分子化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1002/mabi.201000047
语种	英语
WOS记录号	WOS:000281387900017
公开日期	2013-03-07
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/22948]
专题	上海有机化学研究所_高分子材料研究室
推荐引用方式 GB/T 7714	Sheng RL,Luo T,Zhu YD,et al. Interactions of New Synthesized Fluorescent Cationic Amphiphiles Bearing Pyrene Hydrophobe with Plasmid DNA: Binding Affinities, Aggregation and Intracellular Uptake[J]. Macromol. Biosci.,2010,10(8):974-982.
APA	盛瑞隆,罗挺,朱英丹,李慧,&曹阿民.(2010).Interactions of New Synthesized Fluorescent Cationic Amphiphiles Bearing Pyrene Hydrophobe with Plasmid DNA: Binding Affinities, Aggregation and Intracellular Uptake.Macromol. Biosci.,10(8),974-982.
MLA	盛瑞隆,et al."Interactions of New Synthesized Fluorescent Cationic Amphiphiles Bearing Pyrene Hydrophobe with Plasmid DNA: Binding Affinities, Aggregation and Intracellular Uptake".Macromol. Biosci. 10.8(2010):974-982.