A physico-chemical comparative study on extracellular carbohydrate polymers from five desert algae

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	A physico-chemical comparative study on extracellular carbohydrate polymers from five desert algae
	Hokputsa, S ; Hu, CX ; Paulsen, BS ; Harding, SE
刊名	CARBOHYDRATE POLYMERS
	2003-10-01
卷号	54 期号:1 页码:27-32
关键词	desert algal polysaccharide hydrodynamic characterization
ISSN号	0144-8617
通讯作者	Harding, SE, Univ Nottingham, Sch Biosci, NCMH Phys Biochem Lab, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England
中文摘要	Hydrodynamic properties of five newly isolated algal extracellular polysaccharides with putative adhesive properties are described, using a combination of size exclusion chromatography, total or 'multi-angle' laser light scattering and analytical ultracentrifugation. The respective polysaccharides had been extracted from four filamentous cyanobacteria: Microcoleus vaginatus, Scytonema javanicum, Phormidium tenue and Nostoc sp. and a coccoid single-cell green. algae Desmococcus olivaceus that had been separated from desert algal crusts of the Chinese Tegger Desert. SEC/MALLS experiments showed that the saccharides had, diverse-weight average molecular weights ranging from 4000 to 250,000 g/mol and all five showed either bi-modal or tri-modal molecular weight distribution profiles. Use of the Mark-Houwink-Kuhn-Sakurada (MHKS) scaling relationship between sedimentation coefficient and (weight average) molecular weight for the five samples, assuming a homologous conformation series revealed an MHKS b exponent of (0.33 +/- 0.04), suggesting a conformation between that of a stiff rod (b similar to 0.18) and a random coil (b similar to 0.4-0.5), i.e. a 'flexible rod' or 'stiff coil'. (C) 2003 Elsevier Ltd. All rights reserved.
英文摘要	Hydrodynamic properties of five newly isolated algal extracellular polysaccharides with putative adhesive properties are described, using a combination of size exclusion chromatography, total or 'multi-angle' laser light scattering and analytical ultracentrifugation. The respective polysaccharides had been extracted from four filamentous cyanobacteria: Microcoleus vaginatus, Scytonema javanicum, Phormidium tenue and Nostoc sp. and a coccoid single-cell green. algae Desmococcus olivaceus that had been separated from desert algal crusts of the Chinese Tegger Desert. SEC/MALLS experiments showed that the saccharides had, diverse-weight average molecular weights ranging from 4000 to 250,000 g/mol and all five showed either bi-modal or tri-modal molecular weight distribution profiles. Use of the Mark-Houwink-Kuhn-Sakurada (MHKS) scaling relationship between sedimentation coefficient and (weight average) molecular weight for the five samples, assuming a homologous conformation series revealed an MHKS b exponent of (0.33 +/- 0.04), suggesting a conformation between that of a stiff rod (b similar to 0.18) and a random coil (b similar to 0.4-0.5), i.e. a 'flexible rod' or 'stiff coil'. (C) 2003 Elsevier Ltd. All rights reserved.
学科主题	Chemistry ; Applied; Chemistry ; Organic; Polymer Science
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]	Chemistry ; Polymer Science
关键词[WOS]	CRUSTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000185071800004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9660]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Univ Nottingham, Sch Biosci, NCMH Phys Biochem Lab, Loughborough LE12 5RD, Leics, England 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Univ Oslo, Inst Pharm, N-0316 Oslo, Norway
推荐引用方式 GB/T 7714	Hokputsa, S,Hu, CX,Paulsen, BS,et al. A physico-chemical comparative study on extracellular carbohydrate polymers from five desert algae[J]. CARBOHYDRATE POLYMERS,2003,54(1):27-32.
APA	Hokputsa, S,Hu, CX,Paulsen, BS,&Harding, SE.(2003).A physico-chemical comparative study on extracellular carbohydrate polymers from five desert algae.CARBOHYDRATE POLYMERS,54(1),27-32.
MLA	Hokputsa, S,et al."A physico-chemical comparative study on extracellular carbohydrate polymers from five desert algae".CARBOHYDRATE POLYMERS 54.1(2003):27-32.