Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi)

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	Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi)
	Zhang, YA ; Nie, P ; Luo, HY ; Wang, YP ; Sun, YH ; Zhu, ZY
刊名	VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
	2003-09-15
卷号	95 期号:1-2 页码:81-90
关键词	immunoglobulin cDNA sequence light chain Mandarin fish Chinese perch Simperca chuatsi
ISSN号	0165-2427
通讯作者	Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
中文摘要	Immunoglobulin light chain cDNA sequences of a perciform fish, the mandarin fish Siniperca chuatsi were amplified from head kidney mRNA by reverse transcription (RT)-PCR and RACE methods using degenerated primer and gene specific ones. In cDNA sequences of the VL region, nucleotide exchanges were present mainly within CDRs, although a lesser degree of variability was also found in FRs. Moreover, the length of CDRI and CDR3 in the mandarin fish is shorter than in most other fish species. In the middle of S. chuatsi CL region, a microsatellite sequence (AGC)(6-8) was found, which is also present in another perciform species, the spotted wolffish (Anarhichas minor). The comparison of amino acid sequence of the mandarin fish CL domain with those of other vertebrates showed the highest degree of similarity of 94.5% to the spotted wolffish, while the similarity with rainbow trout (Oncorhynchus mykiss) Ig L1 (62.7%) and channel catfish (Ictalurus punctatus) Ig LG (55.9%) isotypes is also higher. However, there is only 50% identity in the VL regions between the mandarin fish and the wolffish. The sequence similarity of the mandarin fish CL domain with those of higher vertebrate did not readily allow it to be classified as kappa or lambda isotype. The phylogenetic analyses also demonstrated that the CL genes of the mandarin fish and most other teleost fish cluster as a separate branch out of the mammal kappa and lambda branches. (C) 2003 Elsevier B.V. All rights reserved.
英文摘要	Immunoglobulin light chain cDNA sequences of a perciform fish, the mandarin fish Siniperca chuatsi were amplified from head kidney mRNA by reverse transcription (RT)-PCR and RACE methods using degenerated primer and gene specific ones. In cDNA sequences of the VL region, nucleotide exchanges were present mainly within CDRs, although a lesser degree of variability was also found in FRs. Moreover, the length of CDRI and CDR3 in the mandarin fish is shorter than in most other fish species. In the middle of S. chuatsi CL region, a microsatellite sequence (AGC)(6-8) was found, which is also present in another perciform species, the spotted wolffish (Anarhichas minor). The comparison of amino acid sequence of the mandarin fish CL domain with those of other vertebrates showed the highest degree of similarity of 94.5% to the spotted wolffish, while the similarity with rainbow trout (Oncorhynchus mykiss) Ig L1 (62.7%) and channel catfish (Ictalurus punctatus) Ig LG (55.9%) isotypes is also higher. However, there is only 50% identity in the VL regions between the mandarin fish and the wolffish. The sequence similarity of the mandarin fish CL domain with those of higher vertebrate did not readily allow it to be classified as kappa or lambda isotype. The phylogenetic analyses also demonstrated that the CL genes of the mandarin fish and most other teleost fish cluster as a separate branch out of the mammal kappa and lambda branches. (C) 2003 Elsevier B.V. All rights reserved.
学科主题	Immunology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology ; Veterinary Sciences
研究领域[WOS]	Immunology ; Veterinary Sciences
关键词[WOS]	CHANNEL CATFISH ; RAINBOW-TROUT ; HEAVY-CHAIN ; GENOMIC ORGANIZATION ; SIBERIAN STURGEON ; NUCLEAR PROTEINS ; ACIPENSER BAERI ; GENES ; L. ; VARIABILITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000185530500009
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9644]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Zhang, YA,Nie, P,Luo, HY,et al. Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi)[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2003,95(1-2):81-90.
APA	Zhang, YA,Nie, P,Luo, HY,Wang, YP,Sun, YH,&Zhu, ZY.(2003).Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi).VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,95(1-2),81-90.
MLA	Zhang, YA,et al."Characterization of cDNA encoding immunoglobulin light chain of the Mandarin fish (Siniperca chuatsi)".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 95.1-2(2003):81-90.