alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120

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	alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120
	Ning, DG; Xu, XD
刊名	MICROBIOLOGY-SGM
	2004-02-01
卷号	150 期号:0 页码:447-453
关键词	CalA ftsZ DNA-binding protein Anabaena
ISSN号	1350-0872
通讯作者	Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.
英文摘要	Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.
学科主题	Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	SP STRAIN PCC-7120 ; PCC 7120 ; DIFFERENTIATION ; EXPRESSION ; SEQUENCE ; PATTERN ; GLYCOLIPIDS ; ENVELOPE
收录类别	SCI
语种	英语
WOS记录号	WOS:000188841500023
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9568]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Ning, DG,Xu, XD. alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120[J]. MICROBIOLOGY-SGM,2004,150(0):447-453.
APA	Ning, DG,&Xu, XD.(2004).alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120.MICROBIOLOGY-SGM,150(0),447-453.
MLA	Ning, DG,et al."alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120".MICROBIOLOGY-SGM 150.0(2004):447-453.