Impacts of elevated CO2 concentration on biochemical composition, carbonic anhydrase, and nitrate reductase activity of freshwater green algae

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	Impacts of elevated CO2 concentration on biochemical composition, carbonic anhydrase, and nitrate reductase activity of freshwater green algae
	Xia, JR ; Gao, KS
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2005-06-01
卷号	47 期号:6 页码:668-675
关键词	carbonic anhydrase Chlamydomonas reinhardtii Chlorella pyrenoidosa CO2 nitrate reductase
ISSN号	1672-9072
通讯作者	Gao, KS, Shantou Univ, Inst Marine Biol, Guangdong 515063, Peoples R China
中文摘要	To investigate the biochemical response of freshwater green algae to elevated CO2 concentrations, Chlorella pyrenoidosa Chick and Chlamydomonas reinhardtii Dang cells were cultured at different CO2 concentrations within the range 3-186 μ mol/L and the biochemical composition, carbonic anhydrase (CA), and nitrate reductase activities of the cells were investigated. Chlorophylls (Chl), carotenoids, carbonhydrate, and protein contents were enhanced to varying extents with increasing CO2 concentration from 3-186 μ mol/L. The CO2 enrichment significantly increased the Chl a/Chl b ratio in Chlorella pyrenoidosa, but not in Chlamydomonas reinhardtii. The CO2 concentration had significant effects on CA and nitrate reductase activity. Elevating CO2 concentration to 186 μ mol/L caused a decline in intracellular and extracellullar CA activity. Nitrate reductase activity, under either light or dark conditions, in C. reinhardtii and C. pyrenoidosa was also significantly decreased with CO2 enrichment. From this study, it can be concluded that CO2 enrichment can affect biochemical composition, CA, and nitrate reductase activity, and that the biochemical response was species dependent.
英文摘要	To investigate the biochemical response of freshwater green algae to elevated CO2 concentrations, Chlorella pyrenoidosa Chick and Chlamydomonas reinhardtii Dang cells were cultured at different CO2 concentrations within the range 3-186 μ mol/L and the biochemical composition, carbonic anhydrase (CA), and nitrate reductase activities of the cells were investigated. Chlorophylls (Chl), carotenoids, carbonhydrate, and protein contents were enhanced to varying extents with increasing CO2 concentration from 3-186 μ mol/L. The CO2 enrichment significantly increased the Chl a/Chl b ratio in Chlorella pyrenoidosa, but not in Chlamydomonas reinhardtii. The CO2 concentration had significant effects on CA and nitrate reductase activity. Elevating CO2 concentration to 186 μ mol/L caused a decline in intracellular and extracellullar CA activity. Nitrate reductase activity, under either light or dark conditions, in C. reinhardtii and C. pyrenoidosa was also significantly decreased with CO2 enrichment. From this study, it can be concluded that CO2 enrichment can affect biochemical composition, CA, and nitrate reductase activity, and that the biochemical response was species dependent.
学科主题	Biochemistry & Molecular Biology; Plant Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Plant Sciences
研究领域[WOS]	Biochemistry & Molecular Biology ; Plant Sciences
关键词[WOS]	DUNALIELLA-SALINA CELLS ; CHLAMYDOMONAS-REINHARDTII ; INORGANIC CARBON ; MARINE-PHYTOPLANKTON ; ATMOSPHERIC CO2 ; GROWTH ; NITROGEN ; PHOTOSYNTHESIS ; ENRICHMENT ; PHOSPHORYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000229518500004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9248]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Shantou Univ, Inst Marine Biol, Guangdong 515063, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Xia, JR,Gao, KS. Impacts of elevated CO2 concentration on biochemical composition, carbonic anhydrase, and nitrate reductase activity of freshwater green algae[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2005,47(6):668-675.
APA	Xia, JR,&Gao, KS.(2005).Impacts of elevated CO2 concentration on biochemical composition, carbonic anhydrase, and nitrate reductase activity of freshwater green algae.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,47(6),668-675.
MLA	Xia, JR,et al."Impacts of elevated CO2 concentration on biochemical composition, carbonic anhydrase, and nitrate reductase activity of freshwater green algae".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 47.6(2005):668-675.