Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection
	Dong, Cai-Wen; Zhang, Yi-Bing; Lu, Ai-Jun; Zhu, Rong; Zhang, Fu-Tie; Zhang, Qi-Ya; Gui, Jian-Fang
刊名	FISH & SHELLFISH IMMUNOLOGY
	2007-04-01
卷号	22 期号:4 页码:380-393
关键词	protein arginine methyltransferase 1 Paralichthys olivaceus Scophthalmus maximus rhabdovirus virus (SMRV) virus infection differential expression antiviral immune response
ISSN号	1050-4648
通讯作者	Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Protein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a vitally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx wash also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response. (c) 2006 Elsevier Ltd. All rights reserved.
英文摘要	Protein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a vitally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx wash also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response. (c) 2006 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	I INTERFERON RECEPTOR ; N-METHYLTRANSFERASE ; PARALICHTHYS-OLIVACEUS ; SUBSTRATE-SPECIFICITY ; CELL-LINE ; JAPANESE FLOUNDER ; MX PROTEINS ; METHYLATION ; PRMT1 ; FAMILY
收录类别	SCI
语种	英语
WOS记录号	WOS:000244247700008
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8700]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Dong, Cai-Wen,Zhang, Yi-Bing,Lu, Ai-Jun,et al. Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2007,22(4):380-393.
APA	Dong, Cai-Wen.,Zhang, Yi-Bing.,Lu, Ai-Jun.,Zhu, Rong.,Zhang, Fu-Tie.,...&Gui, Jian-Fang.(2007).Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection.FISH & SHELLFISH IMMUNOLOGY,22(4),380-393.
MLA	Dong, Cai-Wen,et al."Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection".FISH & SHELLFISH IMMUNOLOGY 22.4(2007):380-393.