N-Alkylpyridinium isotope quaternization for matrix-assisted laser desorption/ionization Fourier transform mass spectrometric analysis of cholesterol and fatty alcohols in human hair

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	N-Alkylpyridinium isotope quaternization for matrix-assisted laser desorption/ionization Fourier transform mass spectrometric analysis of cholesterol and fatty alcohols in human hair
	Wang H(汪航) ; Wang HY(王昊阳) ; Zhang L(张立) ; Zhang J(张菁) ; Guo YL(郭寅龙)
刊名	Anal. Chim. Acta
	2011
卷号	690 期号:1 页码:1-9
ISSN号	0003-2670
其他题名	N-烷基吡啶同位素季铵化反应结合基质辅助激光解析傅里叶变换质谱分析人毛发中的胆固醇和脂肪醇
通讯作者	郭寅龙
英文摘要	Isotope-coded reagents have been developed for labeling of amino acids, phenols and fatty acids, but not for alcohols. In this work, a simple reaction based on direct N-alkylpyridinium isotope quaternization (NAPIQ) was developed for mild derivatization of cholesterol and fatty alcohols. Different from the conventional quaternary reagents with cations on themselves, two simple and charge-neutral reagents: pyridine and d(5)-pyridine directly attached N-cationic tag onto the target compounds in the presence of trifluoromethanesulfonic anhydride (Tf(2)O) without tedious sample preparation. The derivatization completed in 5 min and achieved charge labeling of the target compounds, which significantly improved the detection limits of analytes by 10(3)-folds in further analysis by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS). The use of commercially available d(0)/d(5)-pyridine pairs facilitated isotope-coded chemical derivatization and avoided the use of isotope-labeled internal standards; the excess pyridine did not affect the signals of analytes. Utility of the NAPIQ method was examined in the identification of cholesterol and fatty alcohols in small amount of human hair sample (<0.5 mg). The fluctuation of total cholesterol in human body was profiled during time by quantitatively comparing the different segments of a single strand of hair. This study combines the direct pyridinium quaternization with MALDI-FTMS, which offers a perspective and an alternative tool for the identification and quantification of substances in biological matrix by comparing d(0/)d(5) pairs, especially when isotope-labeled internal standards are unavailable. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	分析化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.aca.2011.02.017
语种	英语
WOS记录号	WOS:000289131500001
公开日期	2013-02-25
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/20040]
专题	上海有机化学研究所_分析化学研究室
推荐引用方式 GB/T 7714	Wang H,Wang HY,Zhang L,et al. N-Alkylpyridinium isotope quaternization for matrix-assisted laser desorption/ionization Fourier transform mass spectrometric analysis of cholesterol and fatty alcohols in human hair[J]. Anal. Chim. Acta,2011,690(1):1-9.
APA	汪航,王昊阳,张立,张菁,&郭寅龙.(2011).N-Alkylpyridinium isotope quaternization for matrix-assisted laser desorption/ionization Fourier transform mass spectrometric analysis of cholesterol and fatty alcohols in human hair.Anal. Chim. Acta,690(1),1-9.
MLA	汪航,et al."N-Alkylpyridinium isotope quaternization for matrix-assisted laser desorption/ionization Fourier transform mass spectrometric analysis of cholesterol and fatty alcohols in human hair".Anal. Chim. Acta 690.1(2011):1-9.