Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus
	Zhou, Guang-Zhou; Gui, Lang; Li, Zheng-Qiu; Yuan, Xiu-Ping; Zhang, Qi-Ya
刊名	JOURNAL OF VIROLOGICAL METHODS
	2008-03-01
卷号	148 期号:1-2 页码:205-210
关键词	fish rhabdoviruses monoclonal antibodies immunocytochemistry flow cytometry
ISSN号	0166-0934
通讯作者	Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Two MAbs (3C7 and 3C9) against flounder Paralichthys olivaceus rhabdovirus (PORV) were generated with hybridoma cell fusion technology and characterized by an indirect enzyme-linked immunosorbent assay, isotype test, Western blot and immunodot analysis and immunofluorescence assay. Isotyping tests demonstrated that both of the two MAbs belonged to IgM subclass. Western blot analysis showed the MAbs reacted with 42, 30, and 22 kDa viral proteins, which were localized within the cytoplasm of PORV-infected grass carp ovary (GCO) cells analyzed by indirect immunofluorescences tests. The MAb 3C7 was also selected at random for detecting virus antigens in the inoculated grass carp tissues by immunohistochemistry assay. Flow cytometry tests showed that at the 36 h postinfection (0.25 PFU/cell), the 23% PORV-infected GCO cells could be distinguished from the uninfected cells with the MAb 3C7. Such MAbs could be useful for diagnosis and potential treatment of viral infection. (C) 2007 Elsevier B.V. All rights reserved.
英文摘要	Two MAbs (3C7 and 3C9) against flounder Paralichthys olivaceus rhabdovirus (PORV) were generated with hybridoma cell fusion technology and characterized by an indirect enzyme-linked immunosorbent assay, isotype test, Western blot and immunodot analysis and immunofluorescence assay. Isotyping tests demonstrated that both of the two MAbs belonged to IgM subclass. Western blot analysis showed the MAbs reacted with 42, 30, and 22 kDa viral proteins, which were localized within the cytoplasm of PORV-infected grass carp ovary (GCO) cells analyzed by indirect immunofluorescences tests. The MAb 3C7 was also selected at random for detecting virus antigens in the inoculated grass carp tissues by immunohistochemistry assay. Flow cytometry tests showed that at the 36 h postinfection (0.25 PFU/cell), the 23% PORV-infected GCO cells could be distinguished from the uninfected cells with the MAb 3C7. Such MAbs could be useful for diagnosis and potential treatment of viral infection. (C) 2007 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
关键词[WOS]	VIRAL HEMORRHAGIC SEPTICEMIA ; SCOPHTHALMUS-MAXIMUS RHABDOVIRUS ; LYMPHOCYSTIS DISEASE VIRUS ; COMPLETE GENOMIC SEQUENCE ; FISH RHABDOVIRUS ; SPRING VIREMIA ; CARP VIRUS ; RABIES ; ASSAY ; DIAGNOSIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000254720500026
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8178]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Guang-Zhou,Gui, Lang,Li, Zheng-Qiu,et al. Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus[J]. JOURNAL OF VIROLOGICAL METHODS,2008,148(1-2):205-210.
APA	Zhou, Guang-Zhou,Gui, Lang,Li, Zheng-Qiu,Yuan, Xiu-Ping,&Zhang, Qi-Ya.(2008).Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus.JOURNAL OF VIROLOGICAL METHODS,148(1-2),205-210.
MLA	Zhou, Guang-Zhou,et al."Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus".JOURNAL OF VIROLOGICAL METHODS 148.1-2(2008):205-210.