A comparison of five extraction methods for extracellular polymeric substances (EPS) from biofilm by using three-dimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy

CORC > 新疆生态与地理研究所 > 中国科学院新疆生态与地理研究所 > 中国科学院新疆生态与地理研究所(2010年以前数据)

	A comparison of five extraction methods for extracellular polymeric substances (EPS) from biofilm by using three-dimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy
	Pan, Xiangliang ; Liu, Jing ; Zhang, Daoyong ; Chen, Xi ; Li, Lanhai ; Song, Wenjuan ; Yang, Jianying
刊名	WATER SA
	2010
卷号	36 期号:1 页码:111-116
关键词	ACTIVATED-SLUDGE ANAEROBIC SLUDGE ORGANIC-MATTER HEAVY-METALS CADMIUM EFFICIENCY BRADFORD BINDING
ISSN号	0378-4738
通讯作者	Pan, XL, Chinese Acad Sci, Key Lab Biogeog & Bioresource Arid Land, Xinjiang Inst Ecol & Geog, Urumqi 830011, Xinjiang, Peoples R China
中文摘要	Two physical methods (centrifugation and ultrasonication) and 3 chemical methods (extraction with EDTA, extraction with formaldehyde, and extraction with formaldehyde plus NaOH) for extraction of EPS from alga-bacteria biofilm were assessed. Pretreatment with ultrasound at low intensity doubled the EPS yield without significant modification of the composition of EPS. Extraction with EDTA or extraction with formaldehyde plus NaOH increased yield by about 1 order of magnitude compared with other methods. However, the protein and polysaccharide content in EPS prepared with EDTA or formaldehyde plus NaOH were low. Two fluorescence peaks belonging to protein-like peaks and 2 fluorescence peaks belonging to humic acid-like substances were found in 3DEEM fluorescence spectra of all the EPS samples prepared using different methods. Fulvic-like fluorescence was detected only in the EPS extracted with formaldehyde plus NaOH. Location of, and fluorescence intensity at, each peak were clearly affected by the extraction methods. Dialysis was also found to be an important factor influencing the yield, composition and fluorescence characteristics of EPS.
学科主题	生物化学与分子生物学
收录类别	SCI
公开日期	2011-08-19
内容类型	期刊论文
源URL	[http://ir.xjlas.org/handle/365004/10000]
专题	新疆生态与地理研究所_中国科学院新疆生态与地理研究所(2010年以前数据)
推荐引用方式 GB/T 7714	Pan, Xiangliang,Liu, Jing,Zhang, Daoyong,et al. A comparison of five extraction methods for extracellular polymeric substances (EPS) from biofilm by using three-dimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy[J]. WATER SA,2010,36(1):111-116.
APA	Pan, Xiangliang.,Liu, Jing.,Zhang, Daoyong.,Chen, Xi.,Li, Lanhai.,...&Yang, Jianying.(2010).A comparison of five extraction methods for extracellular polymeric substances (EPS) from biofilm by using three-dimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy.WATER SA,36(1),111-116.
MLA	Pan, Xiangliang,et al."A comparison of five extraction methods for extracellular polymeric substances (EPS) from biofilm by using three-dimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy".WATER SA 36.1(2010):111-116.