The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)
	Hao, Le; Xie, Ping; Fu, Juan; Li, Guangyu; Xiong, Qian; Li, Huiying
刊名	AQUATIC TOXICOLOGY
	2008-10-20
卷号	90 期号:1 页码:1-7
关键词	Microcystin Glutathione S-transferases Real-time PCR Goldfish Carassius auratus L
ISSN号	0166-445X
通讯作者	Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
中文摘要	The glutathione S-transferases play important roles in the detoxification of microcystin. Core-sequences of three classes of GST (mu, kappa and rho) were cloned from goldfish (Carassius auratus L) i.p. injected with cyanobacterial crude extract at two doses (50 and 200 mu g MC-LReq kg(-1) BW). The relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GST mu was inhibited in intestine at both doses and the transcription of GST kappa was inhibited from 12 to 48 h in kidney at both doses. The decreased transcription of GST rho was detected in all three organs at the high dose. It is suggested that transcription inhibition of GST rho might be significant in MCs toxicity at higher toxin concentration in omnivorous freshwater fish. Alteration in transcription of GSTs stimulated by MCs implicates an increased health risk to fish. (C) 2008 Published by Elsevier B.V.
英文摘要	The glutathione S-transferases play important roles in the detoxification of microcystin. Core-sequences of three classes of GST (mu, kappa and rho) were cloned from goldfish (Carassius auratus L) i.p. injected with cyanobacterial crude extract at two doses (50 and 200 mu g MC-LReq kg(-1) BW). The relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GST mu was inhibited in intestine at both doses and the transcription of GST kappa was inhibited from 12 to 48 h in kidney at both doses. The decreased transcription of GST rho was detected in all three organs at the high dose. It is suggested that transcription inhibition of GST rho might be significant in MCs toxicity at higher toxin concentration in omnivorous freshwater fish. Alteration in transcription of GSTs stimulated by MCs implicates an increased health risk to fish. (C) 2008 Published by Elsevier B.V.
学科主题	Marine & Freshwater Biology; Toxicology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology ; Toxicology
研究领域[WOS]	Marine & Freshwater Biology ; Toxicology
关键词[WOS]	GLUTATHIONE-S-TRANSFERASE ; TROUT ONCORHYNCHUS-MYKISS ; PHYTOPLANKTIVOROUS SILVER CARP ; PLAICE PLEURONECTES-PLATESSA ; HEPATOTOXIN MICROCYSTIN-LR ; HARMFUL ALGAL BLOOMS ; FRESH-WATER FISH ; RED-SEA BREAM ; DANIO-RERIO ; ALPHA-CLASS
收录类别	SCI
资助信息	National Basic Research Program of China [2008CB418101]
语种	英语
WOS记录号	WOS:000260655000001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7972]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Hao, Le,Xie, Ping,Fu, Juan,et al. The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)[J]. AQUATIC TOXICOLOGY,2008,90(1):1-7.
APA	Hao, Le,Xie, Ping,Fu, Juan,Li, Guangyu,Xiong, Qian,&Li, Huiying.(2008).The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus).AQUATIC TOXICOLOGY,90(1),1-7.
MLA	Hao, Le,et al."The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)".AQUATIC TOXICOLOGY 90.1(2008):1-7.