EFFECT OF ELEVATED BICARBONATE CONCENTRATION ON GROWTH, CHLOROPHYLL A FLUORESCENCE AND ULTRA-STRUCTURE OF Microcystis aeruginosa (CYANOBACTERIUM)

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	EFFECT OF ELEVATED BICARBONATE CONCENTRATION ON GROWTH, CHLOROPHYLL A FLUORESCENCE AND ULTRA-STRUCTURE OF Microcystis aeruginosa (CYANOBACTERIUM)
	Kang, Li-juan 1,2; Chen, Xiao-juan 3,4; Pan, Xiao-jie 1,2; Chang, Feng-yi 1,2; Liu, Yong-ding 1
刊名	FRESENIUS ENVIRONMENTAL BULLETIN
	2009
卷号	18 期号:5A 页码:687-693
关键词	HCO(3)(-) Microcystis aeruginosa bicarbonate Chl fluorescence ultrastrcture
ISSN号	1018-4619
通讯作者	Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	The influence of bicarbonate (HCO3-) on Microcystis aeruginosa FACHB 905 was assessed in this study. Growth curves, chlorophyll a fluorescence and ultrastructure were measured at two HCO3- concentrations, 2.3 mM and 12.4 mM. A treatment of sodium chloride (NaCl) was also conducted alongside to establish the influence level of sodium. It was found that upon treatment with elevated HCO3- concentrations of 2.3 mM and 12.4 mM, cell densities were 13% and 27% (respectively) higher than controls. In photosynthetic performance, elevated HCO3- concentration initially stimulated Fv/Fm at the prophase of culture and then subsequently inhibited it. The inhibition of 2.3mM was higher than that of 12.4mM HCO3-. The maximum relative electron transport rate (ETRmax) exhibited inhibition at elevated HCO3- concentrations. DI0/CS was decreased at 2.3 mM and increased at 12.4mM. In the case of both treatments. ABS/CSI TR0/CS, ET0/CS, RC/CS0 and RC/CSm were decreased by elevated HCO3- concentrations, which indicated damage to photosynthetic apparati and an inactivation of a fraction of reaction centers. This point was also proven by ultrastructural photos. High HCO3--exposed cells lost the characteristic photosynthetic membrane arrangement compared with the control and high salinity treated samples. At the 2.3mM concentration of HCO3-. damage to photosynthetic apparati caused decreased photosynthetic activity. These findings suggested that elevated HCO3- concentration stimulated the growth and photosynthesis of M. aeruginosa FACHB 905 in a short time. Exposure to high HCO3- concentrations for a longer period of time will damage photosynthetic apparatus. In addition, the ultrastructure indicated that elevated HCO3--concentration lead to photosynthetic apparati damage. In our experiment, it was observed that the inhibition effect of 2.3mM HCO3- was higher than that of 12.4mM HCO3-. We hypothesized that M. aeruginosa FACHB 905 induced a protective mechanism under high concentrations of HCO3-.
英文摘要	The influence of bicarbonate (HCO(3)(-)) on Microcystis aeruginosa FACHB 905 was assessed in this study. Growth curves, chlorophyll a fluorescence and ultrastructure were measured at two HCO(3)(-) concentrations, 2.3 mM and 12.4 mM. A treatment of sodium chloride (NaCl) was also conducted alongside to establish the influence level of sodium. It was found that upon treatment with elevated HCO(3)(-) concentrations of 2.3 mM and 12.4 mM, cell densities were 13% and 27% (respectively) higher than controls. In photosynthetic performance, elevated HCO(3)(-) concentration initially stimulated Fv/Fm at the prophase of culture and then subsequently inhibited it. The inhibition of 2.3mM was higher than that of 12.4mM HCO(3)(-). The maximum relative electron transport rate (ETRmax) exhibited inhibition at elevated HCO(3)(-) concentrations. DI(0)/CS was decreased at 2.3 mM and increased at 12.4mM. In the case of both treatments. ABS/CSI TR(0)/CS, ET(0)/CS, RC/CS(0) and RC/CS(m) were decreased by elevated HCO(3)(-) concentrations, which indicated damage to photosynthetic apparati and an inactivation of a fraction of reaction centers. This point was also proven by ultrastructural photos. High HCO(3)(-)-exposed cells lost the characteristic photosynthetic membrane arrangement compared with the control and high salinity treated samples. At the 2.3mM concentration of HCO(3)(-). damage to photosynthetic apparati caused decreased photosynthetic activity. These findings suggested that elevated HCO(3)(-) concentration stimulated the growth and photosynthesis of M. aeruginosa FACHB 905 in a short time. Exposure to high HCO(3)(-) concentrations for a longer period of time will damage photosynthetic apparatus. In addition, the ultrastructure indicated that elevated HCO(3)(-)-concentration lead to photosynthetic apparati damage. In our experiment, it was observed that the inhibition effect of 2.3mM HCO(3)(-) was higher than that of 12.4mM HCO(3)(-). We hypothesized that M. aeruginosa FACHB 905 induced a protective mechanism under high concentrations of HCO(3)(-).
学科主题	Environmental Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences
研究领域[WOS]	Environmental Sciences & Ecology
关键词[WOS]	ATMOSPHERIC CARBON-DIOXIDE ; SYNECHOCYSTIS SP PCC-6803 ; RICH IRRIGATION WATERS ; INORGANIC CARBON ; ENERGY-DISSIPATION ; LIGHT ACCLIMATION ; GENE-EXPRESSION ; NUTRIENT-UPTAKE ; PHOTOSYNTHESIS ; PLANTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000266898500005
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7706]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China 4.Chinese Acad Sci, Wuhan 430079, Peoples R China
推荐引用方式 GB/T 7714	Kang, Li-juan,Chen, Xiao-juan,Pan, Xiao-jie,et al. EFFECT OF ELEVATED BICARBONATE CONCENTRATION ON GROWTH, CHLOROPHYLL A FLUORESCENCE AND ULTRA-STRUCTURE OF Microcystis aeruginosa (CYANOBACTERIUM)[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2009,18(5A):687-693.
APA	Kang, Li-juan,Chen, Xiao-juan,Pan, Xiao-jie,Chang, Feng-yi,&Liu, Yong-ding.(2009).EFFECT OF ELEVATED BICARBONATE CONCENTRATION ON GROWTH, CHLOROPHYLL A FLUORESCENCE AND ULTRA-STRUCTURE OF Microcystis aeruginosa (CYANOBACTERIUM).FRESENIUS ENVIRONMENTAL BULLETIN,18(5A),687-693.
MLA	Kang, Li-juan,et al."EFFECT OF ELEVATED BICARBONATE CONCENTRATION ON GROWTH, CHLOROPHYLL A FLUORESCENCE AND ULTRA-STRUCTURE OF Microcystis aeruginosa (CYANOBACTERIUM)".FRESENIUS ENVIRONMENTAL BULLETIN 18.5A(2009):687-693.