Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR

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	Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR
	Huang, Wenmin 1,2; Xing, Wei 3; Li, Dunhai 1; Liu, Yongding 1
刊名	CHEMOSPHERE
	2009-08-01
卷号	76 期号:7 页码:1006-1012
关键词	Microcystin-RR Tobacco BY-2 cells Morphology Ultrastructure Apoptosis Necrosis
ISSN号	0045-6535
通讯作者	Li, DH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China
中文摘要	Tobacco BY-2 cells were exposed to microcystin-RR (MC-RR) at two concentrations, 60 mu g mL(-1) and 120 mu g mL(-1), to study the changes in morphology and ultrastructure of cells as a result of the exposure. Exposure to the lower concentration for 5 d led to typical apoptotic morphological changes including condensation of nuclear chromatin, creation of a characteristic 'half moon' structure, and cytoplasm shrinkage and decreased cell volume, as revealed through light microscopy. fluorescence microscopy, and transmission electron microscopy, respectively. Exposure to the higher concentration, on the other hand, led to morphological and ultrastructural changes typical of necrosis, such as rupture of the plasma membrane and the nuclear membrane and a marked swelling of cells. The presence of many vacuoles containing unusual deposits points to the involvement of vacuoles in detoxifying MC-RR. Results of the present study indicate that exposure of tobacco BY-2 cells to MC-RR at a lower concentration (60 mu g mL(-1)) results in apoptosis and that to a higher concentration (120 mu g mL(-1)), in necrosis. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要	Tobacco BY-2 cells were exposed to microcystin-RR (MC-RR) at two concentrations, 60 mu g mL(-1) and 120 mu g mL(-1), to study the changes in morphology and ultrastructure of cells as a result of the exposure. Exposure to the lower concentration for 5 d led to typical apoptotic morphological changes including condensation of nuclear chromatin, creation of a characteristic 'half moon' structure, and cytoplasm shrinkage and decreased cell volume, as revealed through light microscopy. fluorescence microscopy, and transmission electron microscopy, respectively. Exposure to the higher concentration, on the other hand, led to morphological and ultrastructural changes typical of necrosis, such as rupture of the plasma membrane and the nuclear membrane and a marked swelling of cells. The presence of many vacuoles containing unusual deposits points to the involvement of vacuoles in detoxifying MC-RR. Results of the present study indicate that exposure of tobacco BY-2 cells to MC-RR at a lower concentration (60 mu g mL(-1)) results in apoptosis and that to a higher concentration (120 mu g mL(-1)), in necrosis. (C) 2009 Elsevier Ltd. All rights reserved.
学科主题	Environmental Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences
研究领域[WOS]	Environmental Sciences & Ecology
关键词[WOS]	MITOCHONDRIAL PERMEABILITY TRANSITION ; TROUT ONCORHYNCHUS-MYKISS ; ALBA L. SEEDLINGS ; CYANOBACTERIAL TOXIN ; INDUCED APOPTOSIS ; OXIDATIVE STRESS ; SECONDARY METABOLITES ; SUSPENSION CELLS ; RAT HEPATOCYTES ; AQUATIC PLANTS
收录类别	SCI
资助信息	National Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10]
语种	英语
WOS记录号	WOS:000269297200020
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7604]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, PR, Peoples R China
推荐引用方式 GB/T 7714	Huang, Wenmin,Xing, Wei,Li, Dunhai,et al. Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR[J]. CHEMOSPHERE,2009,76(7):1006-1012.
APA	Huang, Wenmin,Xing, Wei,Li, Dunhai,&Liu, Yongding.(2009).Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR.CHEMOSPHERE,76(7),1006-1012.
MLA	Huang, Wenmin,et al."Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR".CHEMOSPHERE 76.7(2009):1006-1012.