PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status
	Wu, Li 1,2; Yu, Yuhe 1; Zhang, Tanglin 1; Feng, Weisong 1; Zhang, Xiang 1,2; Li, Wei 1,2
刊名	INTERNATIONAL REVIEW OF HYDROBIOLOGY
	2009
卷号	94 期号:5 页码:528-541
关键词	freshwater plankton community DNA analysis 16S rRNA 18S rRNA water quality
ISSN号	1434-2944
通讯作者	Wu, L, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Plankton communities in eight lakes of different trophic status near Yangtze, China were characterized by using denatured gradient gel electrophoresis (DGGE). Various water quality parameters were also measured at each collection site. Following extraction of DNA from plankton communities, 16S rRNA and 18S rRNA genes were amplified with specific primers for prokaryotes and eukaryotes, respectively; DNA profiles were developed by DGGE. The plankton community of each lake had its own distinct DNA profile. The total number of bands identified at 34 sampling stations ranged from 37 to 111. Both prokaryotes and eukaryotes displayed complex fingerprints composed of a large number of bands: 16 to 59 bands were obtained with the prokaryotic primer set; 21 to 52 bands for the eukaryotic primer set. The DGGE-patterns were analyzed in relation to water quality parameters by canonical correspondence analysis (CCA). Temperature, pH, alkalinity, and the concentration of COD, TP and TN were strongly correlated with the DGGE patterns. The parameters that demonstrated a strong correlation to the DGGE fingerprints of the plankton community differed among lakes, suggesting that differences in the DGGE fingerprints were due mainly to lake trophic status. Results of the present study suggest that PCR-DGGE fingerprinting is an effective and precise method of identifying changes to plankton community composition, and therefore could be a useful ecological tool for monitoring the response of aquatic ecosystems to environmental perturbations.
英文摘要	Plankton communities in eight lakes of different trophic status near Yangtze, China were characterized by using denatured gradient gel electrophoresis (DGGE). Various water quality parameters were also measured at each collection site. Following extraction of DNA from plankton communities, 16S rRNA and 18S rRNA genes were amplified with specific primers for prokaryotes and eukaryotes, respectively; DNA profiles were developed by DGGE. The plankton community of each lake had its own distinct DNA profile. The total number of bands identified at 34 sampling stations ranged from 37 to 111. Both prokaryotes and eukaryotes displayed complex fingerprints composed of a large number of bands: 16 to 59 bands were obtained with the prokaryotic primer set; 21 to 52 bands for the eukaryotic primer set. The DGGE-patterns were analyzed in relation to water quality parameters by canonical correspondence analysis (CCA). Temperature, pH, alkalinity, and the concentration of COD, TP and TN were strongly correlated with the DGGE patterns. The parameters that demonstrated a strong correlation to the DGGE fingerprints of the plankton community differed among lakes, suggesting that differences in the DGGE fingerprints were due mainly to lake trophic status. Results of the present study suggest that PCR-DGGE fingerprinting is an effective and precise method of identifying changes to plankton community composition, and therefore could be a useful ecological tool for monitoring the response of aquatic ecosystems to environmental perturbations.
学科主题	Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology
研究领域[WOS]	Marine & Freshwater Biology
关键词[WOS]	GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; ENRICHED SEAWATER MESOCOSMS ; BACTERIAL COMMUNITY ; MICROBIAL COMMUNITY ; GENETIC DIVERSITY ; FRESH-WATER ; ENVIRONMENTAL-FACTORS ; MOLECULAR TECHNIQUES ; SPECIES RICHNESS
收录类别	SCI
资助信息	Major State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016]
语种	英语
WOS记录号	WOS:000271120200002
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7536]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Hefei Normal Univ, Dept Life Sci, Hefei 230061, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wu, Li,Yu, Yuhe,Zhang, Tanglin,et al. PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status[J]. INTERNATIONAL REVIEW OF HYDROBIOLOGY,2009,94(5):528-541.
APA	Wu, Li,Yu, Yuhe,Zhang, Tanglin,Feng, Weisong,Zhang, Xiang,&Li, Wei.(2009).PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status.INTERNATIONAL REVIEW OF HYDROBIOLOGY,94(5),528-541.
MLA	Wu, Li,et al."PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status".INTERNATIONAL REVIEW OF HYDROBIOLOGY 94.5(2009):528-541.