Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 资源植物与生物技术所级重点实验室

	Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis
	Liu, Jin 1,4; Sun, Zheng 2; Zhong, Yujuan 2; Huang, Junchao3 ; Hu, Qiang 4; Chen, Feng 1
刊名	PLANTA
	2012-12-01
卷号	236 期号:6 页码:1665-1676
关键词	Chlorella zofingiensis High light Nitrogen deficiency Oleic acid Stearoyl-acyl carrier protein desaturase
ISSN号	0032-0935
通讯作者	Liu, J (reprint author), Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China.,gjinliu@asu.edu ; sfchencoe@pku.edu.cn
英文摘要	The green alga Chlorella zofingiensis can accumulate high level of oleic acid (OA, C18:1a-(39)) rich oils in response to stress conditions. To understand the regulation of biosynthesis of fatty acid in particular OA at the molecular level, we cloned and characterized the stearoyl acyl carrier protein (ACP) desaturase (SAD) responsible for OA formation through desaturation of stearic acid (C18:0) from C. zofingiensis. Southern blot indicated that the C. zofingiensis genome contained a single copy of SAD, from which the deduced amino acid sequence shared high identity to the corresponding homologs from other microalgae and higher plants. The desaturation activity of SAD was demonstrated in vitro using C18:0-ACP as a substrate. Stress conditions such as high light (HL), nitrogen deficiency (N-), or combination of HL and N- (HL + N-) drastically up-regulated the transcripts of biotin carboxylase (BC, a subunit of ACCase) and SAD, and therefore induced considerably the cellular accumulation of total fatty acids including OA. Glucose (50 mM) gave rise to the similar up-regulation of the two genes and induction of fatty acid accumulation. The accumulation of intracellular reactive oxygen species was found to be associated with the up-regulation of genes. This is the first report of characterization of Chlorella-derived SAD and the results may contribute to understanding of the mechanisms involved in fatty acid/lipid biosynthesis in microalgae.
学科主题	Plant Sciences
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	BIODIESEL PRODUCTION ; TRIACYLGLYCEROL ACCUMULATION ; CHLAMYDOMONAS-REINHARDTII ; NITROGEN DEPRIVATION ; ACID SYNTHESIS ; OLEIC-ACID ; BIOSYNTHESIS ; EXPRESSION ; PROTOTHECOIDES ; ASTAXANTHIN
收录类别	SCI
资助信息	985 Project of Peking University; State Oceanic Administration of China
语种	英语
WOS记录号	WOS:000311398100001
公开日期	2013-01-22
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/15984]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China 2.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China 4.Arizona State Univ Polytech Campus, Dept Appl Sci & Math, Mesa, AZ USA
推荐引用方式 GB/T 7714	Liu, Jin,Sun, Zheng,Zhong, Yujuan,et al. Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis[J]. PLANTA,2012,236(6):1665-1676.
APA	Liu, Jin,Sun, Zheng,Zhong, Yujuan,Huang, Junchao,Hu, Qiang,&Chen, Feng.(2012).Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis.PLANTA,236(6),1665-1676.
MLA	Liu, Jin,et al."Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis".PLANTA 236.6(2012):1665-1676.