Multi-patterned dynamics of mitochondrial fission and fusion in a living cell

	Multi-patterned dynamics of mitochondrial fission and fusion in a living cell
	Wang SQ(王世骐); Xiao WM; Dan SC(单思聪); Jiang CS; Chen M; Zhang Y(章燕); Lv SQ(吕守芹); Chen J(陈娟); Zhang CM; Chen Quan
刊名	PLOS ONE
	2012-05-23
通讯作者邮箱	chenq@ioz.ac.cn;mlong@imech.ac.cn
卷号	7 期号:5 页码:e19879
关键词	Mammalian-Cells Yeast Microtubules Segregation Morphology Apoptosis Transport Movement
ISSN号	1932-6203
通讯作者	Wang, SQ ; Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing, Peoples R China.
产权排序	[Wang, Shiqi;Shan, Sicong; Zhang, Yan; Lu, Shouqin; Chen, Juan; Long, Mian] Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing, Peoples R China; [Wang, Shiqi; Shan, Sicong; Zhang, Yan; Lu, Shouqin; Chen, Juan; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100080, Peoples R China; [Xiao, Weiming; Jiang, Chunsun; Chen, Ming; Chen, Quan] Chinese Acad Sci, Inst Zool, State Key Lab Biomembrane & Membrane Biotechnol, Beijing, Peoples R China; [Xiao, Weiming; Zhang, Chuanmao] Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China; [Xiao, Weiming; Zhang, Chuanmao] Peking Univ, Natl Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China; [Wang, Shiqi] Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Beijing, Peoples R China
合作状况	国内
中文摘要	Mitochondria are highly-dynamic organelles, but it is challenging to monitor quantitatively their dynamics in a living cell. Here we developed a novel approach to determine the global occurrence of mitochondrial fission and fusion events in living human epithelial cells (Hela) and mouse embryonic fibroblast cells (MEF). Distinct patterns of sequential events including fusion followed by fission (Fu-Fi), the so-called "kiss and run'' model previously described, fission followed by fusion (Fi-Fu), fusion followed by fusion (Fu-Fu), and fission followed by fission (Fi-Fi) were observed concurrently. The paired events appeared in high frequencies with short lifetimes and large sizes of individual mitochondria, as compared to those for unpaired events. The high frequencies of paired events were found to be biologically significant. The presence of membrane uncoupler CCCP enhanced the frequency of paired events (from both Fu-Fi and Fi-Fu patterns) with a reduced mitochondrial size. Knock-out of mitofusin protein Mfn1 increased the frequency of fission with increased lifetime of unpaired events whereas deletion of both Mfn1 and Mfn2 resulted in an instable dynamics. These results indicated that the paired events were dominant but unpaired events were not negligible, which provided a new insight into mitochondrial dynamics. In addition to kiss and run model of action, our data suggest that, from a global visualization over an entire cell, multiple patterns of action appeared in mitochondrial fusion and fission.
学科主题	生物力学
分类号	二类/Q1
收录类别	SCI
资助信息	This work was supported by Chinese Academy of Sciences grant KJCX2-YW-L08, National Natural Science (http://english.cas.cn/), Foundation of China grants 30730032 and 30730093 (http://www.nsfc.gov.cn/Portal0/default106.htm), National Key Basic Research Foundation of China grant 2006CB910303 (http://www.most.gov.cn/eng/programmes1/200610/t20061009_36223.htm), and National High Technology Research and Development Program of China grant 2007AA02Z306 (http://www.most.gov.cn/eng/programmes1/index.htm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
原文出处	http://dx.doi.org/10.1371/journal.pone.0019879
语种	英语
WOS记录号	WOS:000305335800001
公开日期	2013-01-18
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/46593]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Wang SQ,Xiao WM,Dan SC,et al. Multi-patterned dynamics of mitochondrial fission and fusion in a living cell[J]. PLOS ONE,2012,7(5):e19879.
APA	王世骐.,Xiao WM.,单思聪.,Jiang CS.,Chen M.,...&龙勉.(2012).Multi-patterned dynamics of mitochondrial fission and fusion in a living cell.PLOS ONE,7(5),e19879.
MLA	王世骐,et al."Multi-patterned dynamics of mitochondrial fission and fusion in a living cell".PLOS ONE 7.5(2012):e19879.