A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters

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	A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters
	Zha, Shihong; Xu, Xudong; Hu, Hanhua
刊名	FEMS MICROBIOLOGY LETTERS
	2012-09-01
卷号	334 期号:2 页码:135-142
关键词	Nostoc sp PCC 7120 bioreporter iron alr0397
ISSN号	0378-1097
通讯作者	Hu, HH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要	A Nostoc sp. PCC 7120 iron bioreporter containing iron-regulated schizokinen transporter gene alr0397 promoter fused to the luxAB genes was examined to optimize its response to bioavailable iron. Doseresponse relationships between luciferase activity and free ferric ion (Fe3+) concentrations pFe (-lg [Fe3+]) were generated by measuring luciferase activities of the bioreporter in trace metalbuffered Fraquil medium with various incubation times. The results were best demonstrated by sigmoidal curves (pFe 18.821.7, Fe3+ = 10-18.810-21.7 M) with the linear range extending from pFe 19.621.5 (Fe3+ = 10-19.610-21.5 M) after a 12-h incubation time. Optimal conditions for the use of this bioreporter to sense the iron bioavailability were determined to be: a 12-h exposure time, initial cell density of OD730 nm = 0.06, high nitrate (100 mu M), high phosphate (10 mu M), moderate Co2+ (0.122.5 nM), Zn2+ (0.1612 nM), Cu2+ (0.0450 nM), and wide range of Mn2+ concentration (0.922300 nM). The applicability of using this iron bioreporter to assess iron availability in the natural environment has been tested using water samples from eutrophic Taihu, Donghu, and Chaohu lakes. It is indicated that the bioreporter is a useful tool to assess bioavailable iron in various water quality samples, especially in eutrophic lakes with high bioavailable iron.
英文摘要	A Nostoc sp. PCC 7120 iron bioreporter containing iron-regulated schizokinen transporter gene alr0397 promoter fused to the luxAB genes was examined to optimize its response to bioavailable iron. Doseresponse relationships between luciferase activity and free ferric ion (Fe3+) concentrations pFe (-lg [Fe3+]) were generated by measuring luciferase activities of the bioreporter in trace metalbuffered Fraquil medium with various incubation times. The results were best demonstrated by sigmoidal curves (pFe 18.821.7, Fe3+ = 10-18.810-21.7 M) with the linear range extending from pFe 19.621.5 (Fe3+ = 10-19.610-21.5 M) after a 12-h incubation time. Optimal conditions for the use of this bioreporter to sense the iron bioavailability were determined to be: a 12-h exposure time, initial cell density of OD730 nm = 0.06, high nitrate (100 mu M), high phosphate (10 mu M), moderate Co2+ (0.122.5 nM), Zn2+ (0.1612 nM), Cu2+ (0.0450 nM), and wide range of Mn2+ concentration (0.922300 nM). The applicability of using this iron bioreporter to assess iron availability in the natural environment has been tested using water samples from eutrophic Taihu, Donghu, and Chaohu lakes. It is indicated that the bioreporter is a useful tool to assess bioavailable iron in various water quality samples, especially in eutrophic lakes with high bioavailable iron.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	STRAIN PCC 7120 ; LAKE-ERIE ; CYANOBACTERIAL BIOREPORTER ; MARINE-PHYTOPLANKTON ; COMPLEXING LIGANDS ; ESCHERICHIA-COLI ; FE ; SYNECHOCOCCUS ; ANABAENA ; PHOSPHORUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000307888400009
公开日期	2013-01-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/17294]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zha, Shihong,Xu, Xudong,Hu, Hanhua. A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters[J]. FEMS MICROBIOLOGY LETTERS,2012,334(2):135-142.
APA	Zha, Shihong,Xu, Xudong,&Hu, Hanhua.(2012).A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters.FEMS MICROBIOLOGY LETTERS,334(2),135-142.
MLA	Zha, Shihong,et al."A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters".FEMS MICROBIOLOGY LETTERS 334.2(2012):135-142.