Liver sinusoid on a chip

doi:10.1016/bs.mcb.2018.06.002

	Liver sinusoid on a chip
	Du Y(杜宇)2,3 ; Li N(李宁)1,2,3 ; Long M(龙勉)1,2,3
刊名	MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS
	2018
卷号	146 页码:105-134
ISSN号	0091-679X
DOI	10.1016/bs.mcb.2018.06.002
通讯作者	Long, Mian(mlong@imech.ac.cn)
英文摘要	Liver sinusoid is the main functional site in liver. Multiple types of hepatic cells are well organized in a precisely-controlled biochemical and biomechanical environment, maintaining a spectrum of hepatic functions. Here, using micro-engineering techniques, four types of primary hepatic cells are integrated into two layer channels connected by porous membrane, which recreates the sinusoidal cell composition and architecture. By incorporating shear flow into this permeable system, the blood flow in sinusoids and interstitial flow in space of Disse are recapitulated. Conventional hepatocyte-based liver-specific functions are enhanced by non-parenchymal cells co-culture and shear flow. Moreover, major immune responses in liver sinusoids, i.e., neutrophil recruitment under lipopolysaccharide (LPS) stimulation, are replicated, indicating that all types of hepatic cells contribute to this process. Thus, this liver chip provides a new in vitro model to investigate the short-duration cellular interactions under a microenvironment mimicking the physiological composition and architecture of liver organ.
分类号	Q4
WOS关键词	ENDOTHELIAL-CELLS ; INFLAMED LIVER ; KUPFFER CELLS ; GENE-EXPRESSION ; CXC CHEMOKINES ; FLOW DYNAMICS ; INJURY ; NEUTROPHILS ; MECHANISMS ; LYMPHOCYTES
WOS研究方向	Cell Biology
语种	英语
WOS记录号	WOS:000452411700007
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/78961]
专题	力学研究所_国家微重力实验室
作者单位	1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing, Peoples R China; 3.Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing, Peoples R China;
推荐引用方式 GB/T 7714	Du Y,Li N,Long M. Liver sinusoid on a chip[J]. MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS,2018,146:105-134.
APA	杜宇,李宁,&龙勉.(2018).Liver sinusoid on a chip.MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS,146,105-134.
MLA	杜宇,et al."Liver sinusoid on a chip".MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS 146(2018):105-134.