Compact type-I coil planet centrifuge for counter-current chromatography | |
Yang Yi; Gu Dongyu; Liu Yongqiang; Aisa Haji Akber; Ito Yoichiro | |
刊名 | Journal of Chromatography A |
2010 | |
卷号 | 1217期号:8页码:1313-1319 |
关键词 | Compact type-I coil planet centrifuge Counter-current chromatography DNP-amino acids Revolution radius Retention of the stationary phase Resolution Retention time |
ISSN号 | 219673 |
英文摘要 | A compact type-I coil planet centrifuge has been developed for performing counter-current chromatography. It has a revolution radius of 10 cm and a column holder height of 5 cm compared with 37 and 50 cm in the original prototype, respectively. The reduction in the revolution radius and column length permits application of higher revolution speed and more stable balancing of the rotor which leads us to learn more about its performance and the future potential of type-I coil planet centrifuge. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate (N) and peak retention time (tR). The results of the experiment indicated that increasing the revolution speed slightly improved both the retention of the stationary phase and the peak resolution while the separation time is remarkably shortened to yield an excellent peak resolution at a revolution speed of 800 rpm. With a 12 ml capacity coiled column, DNP-DL-glu, DNP-β-ala and DNP-l-ala were resolved at Rs of 2.75 and 2.16 within 90 min at a flow rate of 0.4 ml/min. We believe that the compact type-I coil planet centrifuge has a high analytical potential. |
学科主题 | Biochemistry & Molecular Biology ; Chemistry |
WOS记录号 | WOS:000274953400017 |
公开日期 | 2012-11-29 |
内容类型 | 期刊论文 |
源URL | [http://ir.xjipc.cas.cn/handle/365002/1849] |
专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 |
作者单位 | NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Yang Yi,Gu Dongyu,Liu Yongqiang,et al. Compact type-I coil planet centrifuge for counter-current chromatography[J]. Journal of Chromatography A,2010,1217(8):1313-1319. |
APA | Yang Yi,Gu Dongyu,Liu Yongqiang,Aisa Haji Akber,&Ito Yoichiro.(2010).Compact type-I coil planet centrifuge for counter-current chromatography.Journal of Chromatography A,1217(8),1313-1319. |
MLA | Yang Yi,et al."Compact type-I coil planet centrifuge for counter-current chromatography".Journal of Chromatography A 1217.8(2010):1313-1319. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论